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@inproceedings{Miles:2020:10.1183/13993003.congress-2020.604,
author = {Miles, J and Pinkerton, JW and Bonvini, SJ and Dubuis, E and Wortley, MA and Pontis, S and Trevisani, M and Villetti, G and Patacchini, R and Molyneaux, PL and Maher, TM and Birrell, MA and Belvisi, MG},
doi = {10.1183/13993003.congress-2020.604},
pages = {1--2},
publisher = {European Respiratory Society},
title = {Modelling IPF-associated chronic cough: role for oxidative stress?},
url = {http://dx.doi.org/10.1183/13993003.congress-2020.604},
year = {2020}
}

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TY  - CPAPER
AB  - Introduction: Chronic cough is a key symptom that plagues the lives of IPF patients, indeed, it is often the reason the disease is initially diagnosed. The aim of this project was to develop a pre-clinical model of IPF-associated cough and compare the mediator profiles with clinical samples, to aid in the search for effective treatments.Methods: Male guinea pigs (around 325g) were intratracheally dosed with vehicle (saline) or bleomycin (6 USP/kg) and coughs were quantified through manual observation of in-cage video footage, by watching 4.5 hours a day, for 3 weeks. Lung tissue, bronchoalveolar lavage fluid (BALF) and blood was harvested from a parallel group of animals. These were used to assess the extent of fibrosis and measure mediator production (e.g. a marker of oxidative stress: 8-isoprostane). Human BALF was obtained from IPF patients and healthy controls.Results: Spontaneous coughing was recorded in guinea pigs given bleomycin, which was associated with an increase in lung fibrosis and BALF 8-isoprostane (a marker of oxidative stress). We noted similar increases in BALF 8-isoprostane levels in the clinical samples.Conclusions: We have developed a preclinical model to aid in the search for a treatment for IPF-associated coughing. Furthermore, initial data suggests that an increase in oxidative burden (a known trigger of coughing) could be an attractive target.
AU  - Miles,J
AU  - Pinkerton,JW
AU  - Bonvini,SJ
AU  - Dubuis,E
AU  - Wortley,MA
AU  - Pontis,S
AU  - Trevisani,M
AU  - Villetti,G
AU  - Patacchini,R
AU  - Molyneaux,PL
AU  - Maher,TM
AU  - Birrell,MA
AU  - Belvisi,MG
DO  - 10.1183/13993003.congress-2020.604
EP  - 2
PB  - European Respiratory Society
PY  - 2020///
SN  - 0903-1936
SP  - 1
TI  - Modelling IPF-associated chronic cough: role for oxidative stress?
UR  - http://dx.doi.org/10.1183/13993003.congress-2020.604
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000606501401056&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://erj.ersjournals.com/content/56/suppl_64/604
UR  - http://hdl.handle.net/10044/1/88110
ER  -

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