Imperial College London
Dr Tom McKinnon

Dr Tom McKinnon

Faculty of Medicine, Department of Immunology and Inflammation

Senior Lecturer in Immunology and Inflammation
 
 
 
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Contact

 

+44 (0)20 3313 2214t.mckinnon03

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Riddell:2019:10.1002/rth2.12166,
author = {Riddell, A and Vinayagam, S and Gomez, K and Laffan, M and McKinnon, T},
doi = {10.1002/rth2.12166},
journal = {RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS},
pages = {126--135},
title = {Evaluation of von Willebrand factor concentrates by platelet adhesion to collagen using an in vitro flow assay},
url = {http://dx.doi.org/10.1002/rth2.12166},
volume = {3},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundVon Willebrand disease (VWD) results from quantitative or qualitative deficiency of von Willebrand factor (VWF) and is treated using VWFcontaining concentrates. Several studies have compared the function of various VWF containing concentrates however this has not been performed using shear based assays.ObjectivesTo compare the plateletcapture potential of 10 commercially available, plasmaderived VWF concentrates under shear conditions.MethodsVWF containing concentrates were assessed for VWF:Ag, VWF:CB, VWF:RCo, factor VIII:C ADAMTS13 content, VWF multimeric profile and glycan content using lectin binding assays. Freethiol content of each concentrate was investigated using MPB binding assays. An in vitro flow assay was used to determine the ability of each concentrate to mediate platelet capture to collagen.ResultsVWF multimeric analysis revealed reduction of high molecular weight (HMW) forms in four of the concentrates (Alphante, Octanate and Haemoctin, and 8Y). The high MW multimer distribution of the remaining six concentrates (Optivate, Wilate, Fandhi, Wilfactin, Haemate P, and Voncento) was similar to the plasma control. Lectin analysis demonstrated that 8Y had increased amount of Tantigen. Although platelet capture after 5 minutes perfusion was similar for all concentrates; Alphante, Octanate, and Haemoctin, demonstrated the lowest levels of platelet capture after 60 seconds of perfusion. Freethiol content and ADAMTS13 levels varied widely between the concentrates but was not correlated with function.ConclusionAlphanate, Octanate, and Haemoctin, lacked HMW multimers and had the lowest initial platelet capture levels suggesting that the presence of VWF HMW multimers are required for initial platelet deposition.
AU  - Riddell,A
AU  - Vinayagam,S
AU  - Gomez,K
AU  - Laffan,M
AU  - McKinnon,T
DO  - 10.1002/rth2.12166
EP  - 135
PY  - 2019///
SN  - 2475-0379
SP  - 126
TI  - Evaluation of von Willebrand factor concentrates by platelet adhesion to collagen using an in vitro flow assay
T2  - RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
UR  - http://dx.doi.org/10.1002/rth2.12166
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000455653800019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/rth2.12166
UR  - http://hdl.handle.net/10044/1/72077
VL  - 3
ER  -
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