Imperial College London
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ProfessorThomasMeier

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

t.meier Website

 
 
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Location

 

501Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Matthies:2014:10.1038/ncomms6286,
author = {Matthies, D and Zhou, W and Klyszejko, AL and Anselmi, C and Yildiz, Ö and Brandt, K and Müller, V and Faraldo-Gómez, JD and Meier, T},
doi = {10.1038/ncomms6286},
journal = {Nature Communications},
pages = {1--14},
title = {High-resolution structure and mechanism of an F/V-hybrid rotor ring in a Na+-coupled ATP synthase},
url = {http://dx.doi.org/10.1038/ncomms6286},
volume = {5},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - All rotary ATPases catalyse the interconversion of ATP and ADP-Pi through a mechanism that is coupled to the transmembrane flow of H+ or Na+. Physiologically, however, F/A-type enzymes specialize in ATP synthesis driven by downhill ion diffusion, while eukaryotic V-type ATPases function as ion pumps. To begin to rationalize the molecular basis for this functional differentiation, we solved the crystal structure of the Na+-driven membrane rotor of the Acetobacterium woodii ATP synthase, at 2.1 Å resolution. Unlike known structures, this rotor ring is a 9:1 heteromer of F- and V-type c-subunits and therefore features a hybrid configuration of ion-binding sites along its circumference. Molecular and kinetic simulations are used to dissect the mechanisms of Na+ recognition and rotation of this c-ring, and to explain the functional implications of the V-type c-subunit. These structural and mechanistic insights indicate an evolutionary path between synthases and pumps involving adaptations in the rotor ring.
AU  - Matthies,D
AU  - Zhou,W
AU  - Klyszejko,AL
AU  - Anselmi,C
AU  - Yildiz,Ö
AU  - Brandt,K
AU  - Müller,V
AU  - Faraldo-Gómez,JD
AU  - Meier,T
DO  - 10.1038/ncomms6286
EP  - 14
PY  - 2014///
SN  - 2041-1723
SP  - 1
TI  - High-resolution structure and mechanism of an F/V-hybrid rotor ring in a Na+-coupled ATP synthase
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms6286
UR  - https://www.nature.com/articles/ncomms6286#Abs1
UR  - http://hdl.handle.net/10044/1/94433
VL  - 5
ER  -
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