Imperial College London


Faculty of Natural SciencesDepartment of Life Sciences

Honorary Senior Lecturer







Mrs Lucy Collyns +44 (0)20 7594 5395




Sir Alexander Fleming BuildingSouth Kensington Campus






BibTex format

author = {Bernardini, F and Galizi, R and Wunderlich, M and Taxiarchi, C and Kranjc, N and Kyrou, K and Hammond, A and Nolan, T and Lawniczak, MNK and Papathanos, PA and Crisanti, A and Windbichler, N},
doi = {10.1101/151894},
title = {Cross-species Y chromosome function between malaria vectors of the Anopheles gambiae species complex},
url = {},
year = {2017}

RIS format (EndNote, RefMan)

AB - <jats:title>Abstract</jats:title><jats:p>Y chromosome function, structure and evolution is poorly understood in many species including the <jats:italic>Anopheles</jats:italic> genus of mosquitoes, an emerging model system for studying speciation that also represents the major vectors of malaria. While the Anopheline Y had previously been implicated in male mating behavior, recent data from the <jats:italic>Anopheles gambiae</jats:italic> complex suggests that, apart from the putative primary sex-determiner, no other genes are conserved on the Y. Studying the functional basis of the evolutionary divergence of the Y chromosome in the gambiae complex is complicated by complete F1 male hybrid sterility. Here we used an F1xF0 crossing scheme to overcome a severe bottleneck of male hybrid incompatibilities and enabled us to experimentally purify a genetically labelled <jats:italic>A. gambiae</jats:italic> Y chromosome in an <jats:italic>A. arabiensis</jats:italic> background. Whole genome sequencing confirmed that the <jats:italic>A. gambiae</jats:italic> Y retained its original sequence content in the <jats:italic>A. arabiensis</jats:italic> genomic background. In contrast to comparable experiments in <jats:italic>Drosophila</jats:italic>, we find that the presence of a heterospecific Y chromosome has no significant effect on the expression of <jats:italic>A. arabiensis</jats:italic> genes and transcriptional differences can be explained almost exclusively as a direct consequence of transcripts arising from sequence elements present on the <jats:italic>A. gambiae</jats:italic> Y chromosome itself. We find that Y hybrids show no obvious fertility defects and no substantial reduction in male competitiveness. Our results demonstrate that, despite their radically different structure, Y chromosomes of these two species of the gambiae complex that diver
AU - Bernardini,F
AU - Galizi,R
AU - Wunderlich,M
AU - Taxiarchi,C
AU - Kranjc,N
AU - Kyrou,K
AU - Hammond,A
AU - Nolan,T
AU - Lawniczak,MNK
AU - Papathanos,PA
AU - Crisanti,A
AU - Windbichler,N
DO - 10.1101/151894
PY - 2017///
TI - Cross-species Y chromosome function between malaria vectors of the Anopheles gambiae species complex
UR -
ER -