Imperial College London

DrTonyNolan

Faculty of Natural SciencesDepartment of Life Sciences

Honorary Senior Lecturer
 
 
 
//

Contact

 

t.nolan

 
 
//

Assistant

 

Mrs Lucy Collyns +44 (0)20 7594 5395

 
//

Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Hammond:2015:10.1038/nbt.3439,
author = {Hammond, A and Galizi, R and Kyrou, K and Simoni, A and Siniscalchi, C and Katsanos, D and Gribble, M and Baker, D and Marois, E and Russell, S and Burt, A and Windbichler, N and Crisanti, A and Nolan, T},
doi = {10.1038/nbt.3439},
journal = {Nature Biotechnology},
pages = {78--83},
title = {A CRISPR-Cas9 gene drive system-targeting female reproduction in the malaria mosquito vector Anopheles gambiae},
url = {http://dx.doi.org/10.1038/nbt.3439},
volume = {34},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the minimum requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
AU - Hammond,A
AU - Galizi,R
AU - Kyrou,K
AU - Simoni,A
AU - Siniscalchi,C
AU - Katsanos,D
AU - Gribble,M
AU - Baker,D
AU - Marois,E
AU - Russell,S
AU - Burt,A
AU - Windbichler,N
AU - Crisanti,A
AU - Nolan,T
DO - 10.1038/nbt.3439
EP - 83
PY - 2015///
SN - 1087-0156
SP - 78
TI - A CRISPR-Cas9 gene drive system-targeting female reproduction in the malaria mosquito vector Anopheles gambiae
T2 - Nature Biotechnology
UR - http://dx.doi.org/10.1038/nbt.3439
UR - http://hdl.handle.net/10044/1/64946
VL - 34
ER -