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@article{Simoni:2020:10.1038/s41587-020-0508-1,
author = {Simoni, A and Hammond, AM and Beaghton, AK and Galizi, R and Taxiarchi, C and Kyrou, K and Meacci, D and Gribble, M and Morselli, G and Burt, A and Nolan, T and Crisanti, A},
doi = {10.1038/s41587-020-0508-1},
journal = {Nature Biotechnology},
pages = {1054--1060},
title = {A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae},
url = {http://dx.doi.org/10.1038/s41587-020-0508-1},
volume = {38},
year = {2020}
}

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TY  - JOUR
AB  - Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10-14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control.
AU  - Simoni,A
AU  - Hammond,AM
AU  - Beaghton,AK
AU  - Galizi,R
AU  - Taxiarchi,C
AU  - Kyrou,K
AU  - Meacci,D
AU  - Gribble,M
AU  - Morselli,G
AU  - Burt,A
AU  - Nolan,T
AU  - Crisanti,A
DO  - 10.1038/s41587-020-0508-1
EP  - 1060
PY  - 2020///
SN  - 1087-0156
SP  - 1054
TI  - A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae
T2  - Nature Biotechnology
UR  - http://dx.doi.org/10.1038/s41587-020-0508-1
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32393821
UR  - https://www.nature.com/articles/s41587-020-0508-1
UR  - http://hdl.handle.net/10044/1/79258
VL  - 38
ER  -

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