Imperial College London
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DrThomasOuldridge

Faculty of Engineering, Department of Bioengineering

Reader in Biomolecular Systems
 
 
 
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Contact

 

t.ouldridge Website CV

 
 
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Location

 

4.04Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ledesma:2022:10.1126/sciadv.abm5091,
author = {Ledesma, Amaro R and Ouldridge, T and O'Hare, D and Team, I and Baldwin, G},
doi = {10.1126/sciadv.abm5091},
journal = {Science Advances},
title = {Synthetic biology and bioelectrochemical tools for electrogenetic system engineering},
url = {http://dx.doi.org/10.1126/sciadv.abm5091},
volume = {8},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and electrodes), allowing for the direct integration of electronics with biological processes. However, use of electrogenetic systems is limited by poor activity, tunability and standardisation. In this work we developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Finally, we demonstrated electrochemical activation of gene expression in aerobic conditions using a novel, modular bioelectrochemical device. These genetic and electrochemical tools facilitate the design and improve the performance of electrogenetic systems. Furthermore, the genetic design strategies used can be applied to other redox-responsive promoters to further expand the available tools for electrogenetics.
AU  - Ledesma,Amaro R
AU  - Ouldridge,T
AU  - O'Hare,D
AU  - Team,I
AU  - Baldwin,G
DO  - 10.1126/sciadv.abm5091
PY  - 2022///
SN  - 2375-2548
TI  - Synthetic biology and bioelectrochemical tools for electrogenetic system engineering
T2  - Science Advances
UR  - http://dx.doi.org/10.1126/sciadv.abm5091
UR  - https://www.science.org/doi/10.1126/sciadv.abm5091
UR  - http://hdl.handle.net/10044/1/95797
VL  - 8
ER  -
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