Research Interests and Techniques
My lab is interested in how chromatin remodelling and gene regulatory networks drive the maturation and differentiation of neurons in the developing nervous system. When a neuron is born, it still has a long way to go before it is a full functional unit in the nervous system. Much has been learnt about the cellular changes that a neuron undergoes as it matures, however, much less is known about the chromatin remodelling process that are occurring during neuronal maturation. Our recent paper in eLife (Hassan et al., 2020) implicates a component of the condensin complex in this process.
We use the cell-type specific profiling technique TaDa (Southall et al. 2013) to map the transcriptional state, and the genomic binding of regulatory factors, and have also developed CATaDa for profiling chromatin accessibility in vivo in a cell-specific manner (Aughey et al., 2018)
For more information, please see the lab website.
et al., 2021, Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID., Development, Vol:148, Pages:1-16
et al., 2021, Dynamic adult tracheal plasticity drives stem cell adaptation to changes in intestinal homeostasis in Drosophila, Nature Cell Biology, Vol:23, ISSN:1465-7392, Pages:485-496
et al., 2021, FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type specific profiling of protein-DNA interactions in Drosophila melanogaster, G3: Genes, Genomes, Genetics, Vol:11, ISSN:2160-1836, Pages:1-6
et al., 2020, Maintenance of cell fate by the polycomb group gene sex combs extra enables a partial epithelial mesenchymal transition in drosophila., G3: Genes, Genomes, Genetics, Vol:10, ISSN:2160-1836, Pages:1-18