Imperial College London
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Tony D. Southall

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Genetics
 
 
 
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Contact

 

+44 (0)20 7594 5338t.southall

 
 
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Location

 

407Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cattenoz:2016:10.1534/genetics.115.182154,
author = {Cattenoz, PB and Popkova, A and Southall, TD and Aiello, G and Brand, AH and Giangrande, A},
doi = {10.1534/genetics.115.182154},
journal = {Genetics},
pages = {191--219},
title = {Functional conservation of the Glide/Gcm regulatory network controlling glia, hemocyte, and tendon cell differentiation in drosophila},
url = {http://dx.doi.org/10.1534/genetics.115.182154},
volume = {202},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - High-throughput screens allow us to understand how transcription factors trigger developmental processes, including cell specification. A major challenge is identification of their binding sites because feedback loops and homeostatic interactions may mask the direct impact of those factors in transcriptome analyses. Moreover, this approach dissects the downstream signaling cascades and facilitates identification of conserved transcriptional programs. Here we show the results and the validation of a DNA adenine methyltransferase identification (DamID) genome-wide screen that identifies the direct targets of Glide/Gcm, a potent transcription factor that controls glia, hemocyte, and tendon cell differentiation in Drosophila. The screen identifies many genes that had not been previously associated with Glide/Gcm and highlights three major signaling pathways interacting with Glide/Gcm: Notch, Hedgehog, and JAK/STAT, which all involve feedback loops. Furthermore, the screen identifies effector molecules that are necessary for cell-cell interactions during late developmental processes and/or in ontogeny. Typically, immunoglobulin (Ig) domain–containing proteins control cell adhesion and axonal navigation. This shows that early and transiently expressed fate determinants not only control other transcription factors that, in turn, implement a specific developmental program but also directly affect late developmental events and cell function. Finally, while the mammalian genome contains two orthologous Gcm genes, their function has been demonstrated in vertebrate-specific tissues, placenta, and parathyroid glands, begging questions on the evolutionary conservation of the Gcm cascade in higher organisms. Here we provide the first evidence for the conservation of Gcm direct targets in humans. In sum, this work uncovers novel aspects of cell specification and sets the basis for further understanding of the role of conserved Gcm gene regulatory cascades.
AU  - Cattenoz,PB
AU  - Popkova,A
AU  - Southall,TD
AU  - Aiello,G
AU  - Brand,AH
AU  - Giangrande,A
DO  - 10.1534/genetics.115.182154
EP  - 219
PY  - 2016///
SN  - 1943-2631
SP  - 191
TI  - Functional conservation of the Glide/Gcm regulatory network controlling glia, hemocyte, and tendon cell differentiation in drosophila
T2  - Genetics
UR  - http://dx.doi.org/10.1534/genetics.115.182154
UR  - http://hdl.handle.net/10044/1/32817
VL  - 202
ER  -
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