Publications
212 results found
McKenzie Z, Kendall M, Mackay R-M, et al., 2015, Nanoparticles modulate surfactant protein A and D mediated protection against influenza A infection in vitro, Philosophical Transactions B: Biological Sciences, Vol: 370, ISSN: 0962-8436
Chen S, Goode AE, Skepper JN, et al., 2015, Avoiding artefacts during electron microscopy of silver nanomaterials exposed to biological environments, Journal of Microscopy, Vol: 261, Pages: 157-166, ISSN: 1365-2818
Electron microscopy has been applied widely to study the interaction of nanomaterials with proteins, cells and tissues at nanometre scale. Biological material is most commonly embedded in thermoset resins to make it compatible with the high vacuum in the electron microscope. Room temperature sample preparation protocols developed over decades provide contrast by staining cell organelles, and aim to preserve the native cell structure. However, the effect of these complex protocols on the nanomaterials in the system is seldom considered. Any artefacts generated during sample preparation may ultimately interfere with the accurate prediction of the stability and reactivity of the nanomaterials. As a case study, we review steps in the room temperature preparation of cells exposed to silver nanomaterials (AgNMs) for transmission electron microscopy imaging and analysis. In particular, embedding and staining protocols, which can alter the physicochemical properties of AgNMs and introduce artefacts thereby leading to a misinterpretation of silver bioreactivity, are scrutinized. Recommendations are given for the application of cryogenic sample preparation protocols, which simultaneously fix both particles and diffusible ions. By being aware of the advantages and limitations of different sample preparation methods, compromises or selection of different correlative techniques can be made to draw more accurate conclusions about the data. Lay description: With increasing commercialization of silver nanomaterials (AgNMs) comes a concomitant need to understand occupational health, public safety and environmental implications of these materials. Nanoscale studies of the complex bio-nano interface lie at the heart of technical challenges. Despite numerous reports, there is no consensus regarding biological mechanisms enacted by AgNMs. Powerful new electron microscopy techniques can be used to visualize the interaction of the AgNMs with tissues. However, it is extremely difficult to
Nyga A, Hart A, Tetley TD, 2015, Importance of the HIF pathway in cobalt nanoparticle-induced cytotoxicity and inflammation in human macrophages, NANOTOXICOLOGY, Vol: 9, Pages: 905-917, ISSN: 1743-5390
- Author Web Link
- Cite
- Citations: 33
Sweeney S, Berhanu D, Ruenraroengsak P, et al., 2015, Nano-titanium dioxide bioreactivity with human alveolar type-I-like epithelial cells: Investigating crystalline phase as a critical determinant, NANOTOXICOLOGY, Vol: 9, Pages: 482-492, ISSN: 1743-5390
- Author Web Link
- Cite
- Citations: 9
Theodorou IG, Ryan MP, Tetley TD, et al., 2014, Inhalation of silver nanomaterials - seeing the risks, International Journal of Molecular Sciences, Vol: 15, Pages: 23936-23974, ISSN: 1661-6596
Demand for silver engineered nanomaterials (ENMs) is increasing rapidly in optoelectronic and in health and medical applications due to their antibacterial, thermal, electrical conductive, and other properties. The continued commercial up-scaling of ENM production and application needs to be accompanied by an understanding of the occupational health, public safety and environmental implications of these materials. There have been numerous in vitro studies and some in vivo studies of ENM toxicity but their results are frequently inconclusive. Some of the variability between studies has arisen due to a lack of consistency between experimental models, since small differences between test materials can markedly alter their behaviour. In addition, the propensity for the physicochemistry of silver ENMs to alter, sometimes quite radically, depending on the environment they encounter, can profoundly alter their bioreactivity. Consequently, it is important to accurately characterise the materials before use, at the point of exposure and at the nanomaterial-tissue, or “nanobio”, interface, to be able to appreciate their environmental impact. This paper reviews current literature on the pulmonary effects of silver nanomaterials. We focus our review on describing whether, and by which mechanisms, the chemistry and structure of these materials can be linked to their bioreactivity in the respiratory system. In particular, the mechanisms by which the physicochemical properties (e.g., aggregation state, morphology and chemistry) of silver nanomaterials change in various biological milieu (i.e., relevant proteins, lipids and other molecules, and biofluids, such as lung surfactant) and affect subsequent interactions with and within cells will be discussed, in the context not only of what is measured but also of what can be visualized.
Chung KF, Partridge M, Tetley TD, 2014, Abraham ("Abe") Guz: a life devoted to breathing and breathlessness, EUROPEAN RESPIRATORY JOURNAL, Vol: 44, Pages: 1423-1425, ISSN: 0903-1936
Royce SG, Mukherjee D, Cai T, et al., 2014, Modeling population exposures to silver nanoparticles present in consumer products, JOURNAL OF NANOPARTICLE RESEARCH, Vol: 16, ISSN: 1388-0764
- Author Web Link
- Cite
- Citations: 18
Thorley AJ, Ruenraroengsak P, Potter TE, et al., 2014, Critical Determinants of Uptake and Trans location of Nanoparticles by the Human Pulmonary Alveolar Epithelium, ACS NANO, Vol: 8, Pages: 11778-11789, ISSN: 1936-0851
- Author Web Link
- Cite
- Citations: 98
Mukherjee D, Royce SG, Sarkar S, et al., 2014, Modeling in vitro cellular responses to silver nanoparticles, Journal of Toxicology, Vol: 2014, ISSN: 1687-8205
Engineered nanoparticles (NPs) have been widely demonstrated to induce toxic effects to various cell types. In vitro cell exposure systems have high potential for reliable, high throughput screening of nanoparticle toxicity, allowing focusing on particular pathways while excluding unwanted effects due to other cells or tissue dosimetry. The work presented here involves a detailed biologically based computational model of cellular interactions with NPs; it utilizes measurements performed in human cell culture systems in vitro, to develop a mechanistic mathematical model that can support analysis and prediction of in vivo effects of NPs. The model considers basic cellular mechanisms including proliferation, apoptosis, and production of cytokines in response to NPs. This new model is implemented for macrophages and parameterized using in vitro measurements of changes in cellular viability and mRNA levels of cytokines: TNF, IL-1b, IL-6, IL-8, and IL-10. The model includes in vitro cellular dosimetry due to nanoparticle transport and transformation. Furthermore, the model developed here optimizes the essential cellular parameters based on in vitro measurements, and provides a "stepping stone" for the development of more advanced in vivo models that will incorporate additional cellular and NP interactions.
Mukherjee D, Leo BF, Royce SG, et al., 2014, Modeling physicochemical interactions affecting in vitro cellular dosimetry of engineered nanomaterials: application to nanosilver, JOURNAL OF NANOPARTICLE RESEARCH, Vol: 16, ISSN: 1388-0764
- Author Web Link
- Cite
- Citations: 21
Ruenraroengsak P, Cheng S, Sheng H, et al., 2014, Translocation of carbon nanotubes across the alveolar epithelial barrier, Publisher: EUROPEAN RESPIRATORY SOC JOURNALS LTD, ISSN: 0903-1936
Seiffert J, Hussain F, Guo C, et al., 2014, Inhaled silver nanoparticles induce pulmonary oxidative injury and inflammation: Differential effects between rat strains, Publisher: EUROPEAN RESPIRATORY SOC JOURNALS LTD, ISSN: 0903-1936
- Author Web Link
- Cite
- Citations: 3
Smyth E, Solomon A, Vydyanath A, et al., 2014, Induction and enhancement of platelet aggregation in vitro and in vivo by model polystyrene nanoparticles, Nanotoxicology, Vol: 9, Pages: 356-364, ISSN: 1743-5404
Abstract Nanoparticles (NPs) may come into contact with circulating blood elements including platelets following inhalation and translocation from the airways to the bloodstream or during proposed medical applications. Studies with model polystyrene latex nanoparticles (PLNPs) have shown that NPs are able to induce platelet aggregation in vitro suggesting a poorly defined potential mechanism of increased cardiovascular risk upon NP exposure. We aimed to provide insight into the mechanisms by which NPs may increase cardiovascular risk by determining the impact of a range of concentrations of PLNPs on platelet activation in vitro and in vivo and identifying the signaling events driving NP-induced aggregation. Model PLNPs of varying nano-size (50 and 100 nm) and surface chemistry [unmodified (uPLNP), amine-modified (aPLNP) and carboxyl-modified (cPLNP)] were therefore examined using in vitro platelet aggregometry and an established mouse model of platelet thromboembolism. Most PLNPs tested induced GPIIb/IIIa-mediated platelet aggregation with potencies that varied with both surface chemistry and nano-size. Aggregation was associated with signaling events, such as granule secretion and release of secondary agonists, indicative of conventional agonist-mediated aggregation. Platelet aggregation was associated with the physical interaction of PLNPs with the platelet membrane or internalization. 50 nm aPLNPs acted through a distinct mechanism involving the physical bridging of adjacent non-activated platelets leading to enhanced agonist-induced aggregation in vitro and in vivo. Our study suggests that should they translocate the pulmonary epithelium, or be introduced into the blood, NPs may increase the risk of platelet-driven events by inducing or enhancing platelet aggregation via mechanisms that are determined by their distinct combination of nano-size and surface chemistry.
Sweeney S, Berhanu D, Misra SK, et al., 2014, Multi-walled carbon nanotube length as a critical determinant of bioreactivity with primary human pulmonary alveolar cells., Carbon
Multiwalled carbon nanotube (MWCNT) length is suggested to critically determine their pulmonary toxicity. This stems from in vitro and in vivo rodent studies and in vitro human studies using cell lines (typically cancerous). There is little data using primary human lung cells. We addressed this knowledge gap, using highly relevant, primary human alveolar cell models exposed to precisely synthesized and thoroughly characterized MWCNTs. In this work, transformed human alveolar type-I-like epithelial cells (TT1), primary human alveolar type-II epithelial cells (ATII) and alveolar macrophages (AM) were treated with increasing concentrations of MWCNTs before measuring cytotoxicity, inflammatory mediator release and MAP kinase signalling. Strikingly, we observed that short MWCNTs (∼0.6 μm in length) induced significantly greater responses from the epithelial cells, whilst AM were particularly susceptible to long MWCNTs (∼20 μm). These differences in the pattern of mediator release were associated with alternative profiles of JNK, p38 and ERK1/2 MAP kinase signal transduction within each cell type. This study, using highly relevant target human alveolar cells and well defined and characterized MWCNTs, shows marked cellular responses to the MWCNTs that vary according to the target cell type, as well as the aspect ratio of the MWCNT.
Chen S, Hu S, Smith EF, et al., 2014, Aqueous cationic, anionic and non-ionic multi-walled carbon nanotubes, functionalised with minimal framework damage, for biomedical application, Biomaterials, Vol: 35, Pages: 4729-4738
Misra SK, Nuseibeh S, Dybowska A, et al., 2014, Comparative study using spheres, rods and spindle-shaped nanoplatelets on dispersion stability, dissolution and toxicity of CuO nanomaterials, NANOTOXICOLOGY, Vol: 8, Pages: 422-432, ISSN: 1743-5390
- Author Web Link
- Cite
- Citations: 63
Sarkar S, Zhang L, Subramaniam P, et al., 2014, Variability in Bioreactivity Linked to Changes in Size and Zeta Potential of Diesel Exhaust Particles in Human Immune Cells, PLOS One, Vol: 9, ISSN: 1932-6203
Acting as fuel combustion catalysts to increase fuel economy, cerium dioxide (ceria, CeO2) nanoparticles have been used inEurope as diesel fuel additives (EnviroxTM). We attempted to examine the effects of particles emitted from a diesel engineburning either diesel (diesel exhaust particles, DEP) or diesel doped with various concentrations of CeO2 (DEP-Env) on innateimmune responses in THP-1 and primary human peripheral blood mononuclear cells (PBMC). Batches of DEP and DEP-Envwere obtained on three separate occasions using identical collection and extraction protocols with the aim of determiningthe reproducibility of particles generated at different times. However, we observed significant differences in size and surfacecharge (zeta potential) of the DEP and DEP-Env across the three batches. We also observed that exposure of THP-1 cells andPBMC to identical concentrations of DEP and DEP-Env from the three batches resulted in statistically significant differencesin bioreactivity as determined by IL-1b, TNF-a, IL-6, IFN-c, and IL-12p40 mRNA (by qRT-PCR) and protein expression (byELISPOT assays). Importantly, bioreactivity was noted in very tight ranges of DEP size (60 to 120 nm) and zeta potential (237to 241 mV). Thus, these physical properties of DEP and DEP-Env were found to be the primary determinants of thebioreactivity measured in this study. Our findings also point to the potential risk of over- or under- estimation of expectedbioreactivity effects (and by inference of public health risks) from bulk DEP use without taking into account potential batchto-batchvariations in physical (and possibly chemical) properties.
Schwander S, Zhang JJ, Tetley T, et al., 2014, Silver nanoparticles impair human antimicrobial immune responses, JOURNAL OF IMMUNOLOGY, Vol: 192, ISSN: 0022-1767
Novak P, Shevchuk A, Ruenraroengsak P, et al., 2014, Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy, Nano Letters: a journal dedicated to nanoscience and nanotechnology, Vol: 14, Pages: 1202-1207, ISSN: 1530-6984
Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle–cell interactions.
Serrano-Mollar A, Gay-Jordi G, Guillamat-Prats R, et al., 2014, Alveolar Type Ii Cell Infusion: A Novel Strategy In The Treatment Of Idiopathic Pulmonary Fibrosis, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 189, ISSN: 1073-449X
Chen S, Theodorou IG, Goode AE, et al., 2013, High-Resolution Analytical Electron Microscopy Reveals Cell Culture Media-Induced Changes to the Chemistry of Silver Nanowires, ENVIRONMENTAL SCIENCE & TECHNOLOGY, Vol: 47, Pages: 13813-13821, ISSN: 0013-936X
- Author Web Link
- Cite
- Citations: 27
Nestorowa S, Tetley TD, Thorley AJ, 2013, PULMONARY SURFACTANT PROTECTS AGAINST SILVER NANOPARTICLE-INDUCED INFLAMMATION IN THE PERIPHERAL HUMAN LUNG, Winter Meeting of the British-Thoracic-Society, Publisher: BMJ PUBLISHING GROUP, Pages: A138-A139, ISSN: 0040-6376
Zambianchi MZ, Tetley TD, Thorley AJ, 2013, ALVEOLAR EPITHELIAL DNA DAMAGE, INFLAMMATION AND ALTERED AUTOPHAGY FOLLOWING EXPOSURE TO SILVER NANOPARTICLES IS EXACERBATED BY VIRAL LIGANDS <i>IN VITRO</i>, Winter Meeting of the British-Thoracic-Society, Publisher: BMJ PUBLISHING GROUP, Pages: A52-A52, ISSN: 0040-6376
- Author Web Link
- Cite
- Citations: 2
Zhang J, Nazarenko Y, Zhang L, et al., 2013, Impacts of a Nanosized Ceria Additive on Diesel Engine Emissions of Particulate and Gaseous Pollutants, ENVIRONMENTAL SCIENCE & TECHNOLOGY, Vol: 47, Pages: 13077-13085, ISSN: 0013-936X
- Author Web Link
- Cite
- Citations: 60
Thorley AJ, Tetley TD, 2013, New perspectives in nanomedicine, PHARMACOLOGY & THERAPEUTICS, Vol: 140, Pages: 176-185, ISSN: 0163-7258
- Author Web Link
- Cite
- Citations: 111
Seiffert J, Weigman C, Feng L, et al., 2013, Determinants of inflammatory and functional toxicity of silver nanospheres in rat lung, EUROPEAN RESPIRATORY JOURNAL, Vol: 42, ISSN: 0903-1936
Leo BF, Chen S, Kyo Y, et al., 2013, The Stability of Silver Nanoparticles in a Model of Pulmonary Surfactant., Environ Sci Technol, Vol: 47, Pages: 11232-11240
The growing use of silver nanoparticles (AgNPs) in consumer products has raised concerns about their potential impact on the environment and human health. Whether AgNPs dissolve and release Ag+ ions, or coarsen to form large aggregates, is critical in determining their potential toxicity. In this work, the stability of AgNPs in dipalmitoylphosphatidylcholine (DPPC), the major component of pulmonary surfactant, was investigated as a function of pH. Spherical, citrate-capped AgNPs with average diameters of 14 ± 1.6 nm (n=200) were prepared by a chemical bath reduction. The kinetics of Ag+ ion release was strongly pH-dependent. After 14 days of incubation in sodium perchlorate (NaClO4) or perchloric acid (HClO4) solutions, the total fraction of AgNPs dissolved varied from ~10 % at pH 3, to ~2 % at pH 5, with negligible dissolution at pH 7. A decrease in pH from 7 to 3 also promoted particle aggregation and coarsening. DPPC (100 mg.L-1) delayed the release of Ag+ ions, but did not significantly alter the total amount of Ag+ released after two weeks. In addition, DPPC improved the dispersion of the AgNPs and inhibited aggregation and coarsening. TEM images revealed that the AgNPs were coated with a DPPC layer serving as a semi-permeable layer. Hence, lung lining fluid, particularly DPPC, can modify the aggregation state and kinetics of Ag+ ion release of inhaled AgNPs in the lung. These observations have important implications for predicting the potential reactivity of AgNPs in the lung and the environment.
Damby DE, Horwell CJ, Baxter PJ, et al., 2013, The respiratory health hazard of tephra from the 2010 Centennial eruption of Merapi with implications for occupational mining of deposits, JOURNAL OF VOLCANOLOGY AND GEOTHERMAL RESEARCH, Vol: 261, Pages: 376-387, ISSN: 0377-0273
- Author Web Link
- Cite
- Citations: 44
Emerson M, Solomon A, Smyth E, et al., 2013, Role of platelets in driving the thrombotic risk and protective processes associated with exposure to diesel exhaust particles, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 11, Pages: 643-644, ISSN: 1538-7933
Smyth E, Solomon A, Vydyanath A, et al., 2013, The potencies and mechanisms by which engineered nanoparticles induce platelet aggregation are dependent upon their precise physicochemistry, JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 11, Pages: 895-896, ISSN: 1538-7933
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.