Imperial College London
Portrait Picture

DrTeresaThurston

Faculty of Medicine, Department of Infectious Disease

Senior Lecturer in Molecular Microbiology
 
 
 
//

Contact

 

+44 (0)20 7594 3072t.thurston

 
 
//

Location

 

2.40Flowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Bourgeois:2018:10.1128/IAI.00429-18,
author = {Bourgeois, JS and Zhou, D and Thurston, TLM and Gilchrist, JJ and Ko, DC},
doi = {10.1128/IAI.00429-18},
journal = {Infection and Immunity},
title = {Methylthioadenosine suppresses Salmonella virulence},
url = {http://dx.doi.org/10.1128/IAI.00429-18},
volume = {86},
year = {2018}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In order to deploy virulence factors at appropriate times and locations, microbes must rapidly sense and respond to various metabolite signals. Previously we showed transient elevation of the methionine-derived metabolite methylthioadenosine (MTA) in serum during systemic Salmonella enterica serovar Typhimurium (S. Typhimurium) infection. Here we explored the functional consequences of increased MTA concentrations on S. Typhimurium virulence. We found that MTA-but not other related metabolites involved in polyamine synthesis and methionine salvage-reduced motility, host cell pyroptosis, and cellular invasion. Further, we developed a genetic model of increased bacterial endogenous MTA production by knocking out the master repressor of the methionine regulon, metJ Like MTA-treated S. Typhimurium, the ΔmetJ mutant displayed reduced motility, host cell pyroptosis, and invasion. These phenotypic effects of MTA correlated with suppression of flagellar and Salmonella pathogenicity island-1 (SPI-1) networks. ΔmetJ S. Typhimurium had reduced virulence in oral and intraperitoneal infection of C57BL/6J mice, independently of the effects of MTA on SPI-1. Finally, ΔmetJ bacteria induced a less severe inflammatory cytokine response in a mouse sepsis model. Together, these data indicate that exposure of S. Typhimurium to MTA or disruption of the bacterial methionine metabolism pathway suppresses S. Typhimurium virulence.
AU  - Bourgeois,JS
AU  - Zhou,D
AU  - Thurston,TLM
AU  - Gilchrist,JJ
AU  - Ko,DC
DO  - 10.1128/IAI.00429-18
PY  - 2018///
SN  - 0019-9567
TI  - Methylthioadenosine suppresses Salmonella virulence
T2  - Infection and Immunity
UR  - http://dx.doi.org/10.1128/IAI.00429-18
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29866910
UR  - http://hdl.handle.net/10044/1/62765
VL  - 86
ER  -
Download