Imperial College London
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DrTeresaThurston

Faculty of Medicine, Department of Infectious Disease

Senior Lecturer in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 3072t.thurston

 
 
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Location

 

2.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Switzer:2022:10.1128/jb.00031-22,
author = {Switzer, A and Burchell, L and Mitsidis, P and Thurston, T and Wigneshweraraj, S},
doi = {10.1128/jb.00031-22},
journal = {Journal of Bacteriology},
pages = {1--16},
title = {A role for the RNA polymerase gene specificity factor sigma(54) in the uniform colony growth of uropathogenic escherichia coli},
url = {http://dx.doi.org/10.1128/jb.00031-22},
volume = {204},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The canonical function of a bacterial sigma (σ) factor is to determine the gene specificity of the RNA polymerase (RNAP). In several diverse bacterial species, the σ54 factor uniquely confers distinct functional and regulatory properties on the RNAP. A hallmark feature of the σ54-RNAP is the obligatory requirement for an activator ATPase to allow transcription initiation. Different activator ATPases couple diverse environmental cues to the σ54-RNAP to mediate adaptive changes in gene expression. Hence, the genes that rely upon σ54 for their transcription have a wide range of different functions suggesting that the repertoire of functions performed by genes, directly or indirectly affected by σ54, is not yet exhaustive. By comparing the growth patterns of prototypical enteropathogenic, uropathogenic, and nonpathogenic Escherichia coli strains devoid of σ54, we uncovered that the absence of σ54 results in two differently sized colonies that appear at different times specifically in the uropathogenic E. coli (UPEC) strain. Notably, UPEC bacteria devoid of individual activator ATPases of the σ54-RNAP do not phenocopy the σ54 mutant strain. Thus, it seems that σ54’s role as a determinant of uniform colony appearance in UPEC bacteria represents a putative non-canonical function of σ54 in regulating genetic information flow.
AU  - Switzer,A
AU  - Burchell,L
AU  - Mitsidis,P
AU  - Thurston,T
AU  - Wigneshweraraj,S
DO  - 10.1128/jb.00031-22
EP  - 16
PY  - 2022///
SN  - 0021-9193
SP  - 1
TI  - A role for the RNA polymerase gene specificity factor sigma(54) in the uniform colony growth of uropathogenic escherichia coli
T2  - Journal of Bacteriology
UR  - http://dx.doi.org/10.1128/jb.00031-22
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000783979300029&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.asm.org/doi/10.1128/jb.00031-22
UR  - http://hdl.handle.net/10044/1/99134
VL  - 204
ER  -
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