Imperial College London
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ProfessorThomasChurcher

Faculty of Medicine, School of Public Health

Professor of Infectious Disease Dynamics
 
 
 
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Contact

 

thomas.churcher

 
 
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Location

 

G35Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sanz:2019:10.3389/fcimb.2019.00238,
author = {Sanz, S and Aquilini, E and Tweedell, RE and Verma, G and Hamerly, T and Hritzo, B and Tripathi, A and Machado, M and Churcher, TS and Rodrigues, JA and Izquierdo, L and Dinglasan, RR},
doi = {10.3389/fcimb.2019.00238},
journal = {Frontiers in Cellular and Infection Microbiology},
pages = {1--9},
title = {Protein O-fucosyltransferase 2 is not essential for plasmodium berghei development},
url = {http://dx.doi.org/10.3389/fcimb.2019.00238},
volume = {9},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Thrombospondin type I repeat (TSR) domains are commonly O-fucosylated by protein O-fucosyltransferase 2 (PoFUT2), and this modification is required for optimal folding and secretion of TSR-containing proteins. The human malaria parasite Plasmodium falciparum expresses proteins containing TSR domains, such as the thrombospondin-related anonymous protein (TRAP) and circumsporozoite surface protein (CSP), which are O-fucosylated. TRAP and CSP are present on the surface of sporozoites and play essential roles in mosquito and human host invasion processes during the transmission stages. Here, we have generated PoFUT2 null-mutant P. falciparum and Plasmodium berghei (rodent) malaria parasites and, by phenotyping them throughout their complete life cycle, we show that PoFUT2 disruption does not affect the growth through the mosquito stages for both species. However, contrary to what has been described previously by others, P. berghei PoFUT2 null mutant sporozoites showed no deleterious motility phenotypes and successfully established blood stage infection in mice. This unexpected result indicates that the importance of O-fucosylation of TSR domains may differ between human and RODENT malaria parasites; complicating our understanding of glycosylation modifications in malaria biology.
AU  - Sanz,S
AU  - Aquilini,E
AU  - Tweedell,RE
AU  - Verma,G
AU  - Hamerly,T
AU  - Hritzo,B
AU  - Tripathi,A
AU  - Machado,M
AU  - Churcher,TS
AU  - Rodrigues,JA
AU  - Izquierdo,L
AU  - Dinglasan,RR
DO  - 10.3389/fcimb.2019.00238
EP  - 9
PY  - 2019///
SN  - 2235-2988
SP  - 1
TI  - Protein O-fucosyltransferase 2 is not essential for plasmodium berghei development
T2  - Frontiers in Cellular and Infection Microbiology
UR  - http://dx.doi.org/10.3389/fcimb.2019.00238
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000473670500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.frontiersin.org/articles/10.3389/fcimb.2019.00238/full
UR  - http://hdl.handle.net/10044/1/72168
VL  - 9
ER  -
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