Imperial College London
Alternate

Emeritus ProfessorTimothyWilliams

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor in Airway Disease
 
 
 
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Contact

 

+44 (0)20 7594 3159tim.williams Website

 
 
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Location

 

Office no. 360Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Weller:2005:10.1084/jem.20042407,
author = {Weller, CL and Collington, SJ and Brown, JK and Miller, HRP and Al-Kashi, A and Clark, P and Jose, PJ and Hartnell, A and Williams, TJ},
doi = {10.1084/jem.20042407},
journal = {Journal of Experimental Medicine},
pages = {1961--1971},
title = {Leukotriene B-4, an activation product of mast cells, is a chemoattractant for their progenitors},
url = {http://dx.doi.org/10.1084/jem.20042407},
volume = {201},
year = {2005}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Mast cells are tissue-resident cells with important functions in allergy and inflammation. Pluripotential hematopoietic stem cells in the bone marrow give rise to committed mast cell progenitors that transit via the blood to tissues throughout the body, where they mature. Knowledge is limited about the factors that release mast cell progenitors from the bone marrow or recruit them to remote tissues. Mouse femoral bone marrow cells were cultured with IL-3 for 2 wk and a range of chemotactic agents were tested on the c-kit+ population. Cells were remarkably refractory and no chemotaxis was induced by any chemokines tested. However, supernatants from activated mature mast cells induced pronounced chemotaxis, with the active principle identified as leukotriene (LT) B4. Other activation products were inactive. LTB4 was highly chemotactic for 2-wk-old cells, but not mature cells, correlating with a loss of mRNA for the LTB4 receptor, BLT1. Immature cells also accumulated in vivo in response to intradermally injected LTB4. Furthermore, LTB4 was highly potent in attracting mast cell progenitors from freshly isolated bone marrow cell suspensions. Finally, LTB4 was a potent chemoattractant for human cord blood–derived immature, but not mature, mast cells. These results suggest an autocrine role for LTB4 in regulating tissue mast cell numbers.
AU  - Weller,CL
AU  - Collington,SJ
AU  - Brown,JK
AU  - Miller,HRP
AU  - Al-Kashi,A
AU  - Clark,P
AU  - Jose,PJ
AU  - Hartnell,A
AU  - Williams,TJ
DO  - 10.1084/jem.20042407
EP  - 1971
PY  - 2005///
SN  - 1540-9538
SP  - 1961
TI  - Leukotriene B-4, an activation product of mast cells, is a chemoattractant for their progenitors
T2  - Journal of Experimental Medicine
UR  - http://dx.doi.org/10.1084/jem.20042407
UR  - http://hdl.handle.net/10044/1/28130
VL  - 201
ER  -
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