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@article{Kumar:2007,
author = {Kumar, S and Dunsby, C and De, Beule PAA and Owen, DM and Anand, U and Lanigan, PMP and Benninger, RKP and Davis, DM and Neil, MAA and Anand, P and Benham, C and Naylor, A and French, PMW},
journal = {Optics Express},
pages = {12548--12561},
title = {Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging},
volume = {15},
year = {2007}
}

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TY  - JOUR
AB  - We report a multifocal multiphoton time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode PMT detector.  Multiphoton excitation minimizes out-of-focus photobleaching, multifocal excitation reduces non-linear in-plane photobleaching effects and TCSPC electronics provide photon-efficient detection of the fluorescence decay profile. TCSPC detection is less prone to bleaching- and movementinduced  artefacts compared to wide-field time-gated or frequency-domain FLIM. This microscope is therefore capable of acquiring 3-D FLIM  images at significantly increased speeds compared to single beam multiphoton microscopy and we demonstrate this with live cells expressing a GFP tagged protein. We also apply this system to time-lapse FLIM of NAD(P)H autofluorescence in single live cells and report measurements on the change in the fluorescence decay profile following the application of a  known metabolic inhibitor.
AU  - Kumar,S
AU  - Dunsby,C
AU  - De,Beule PAA
AU  - Owen,DM
AU  - Anand,U
AU  - Lanigan,PMP
AU  - Benninger,RKP
AU  - Davis,DM
AU  - Neil,MAA
AU  - Anand,P
AU  - Benham,C
AU  - Naylor,A
AU  - French,PMW
EP  - 12561
PY  - 2007///
SP  - 12548
TI  - Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging
T2  - Optics Express
VL  - 15
ER  -

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