Imperial College London
Alternate

DrUmaAnand

Faculty of Medicine, Department of Surgery & Cancer

Research Fellow
 
 
 
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Contact

 

+44 (0)20 3313 2362u.anand

 
 
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Location

 

BN5Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sánchez:2008:10.1529/biophysj.108.129551,
author = {Sánchez, D and Johnson, N and Li, C and Novak, P and Rheinlaender, J and Zhang, Y and Anand, U and Anand, P and Gorelik, J and Frolenkov, GI and Benham, C and Lab, M and Ostanin, VP and Schäffer, TE and Klenerman, D and Korchev, YE},
doi = {10.1529/biophysj.108.129551},
journal = {Biophysical journal},
pages = {3017--27},
title = {Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette.},
url = {http://dx.doi.org/10.1529/biophysj.108.129551},
volume = {95},
year = {2008}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Mechanosensitivity in living biological tissue is a study area of increasing importance, but investigative tools are often inadequate. We have developed a noncontact nanoscale method to apply quantified positive and negative force at defined positions to the soft responsive surface of living cells. The method uses applied hydrostatic pressure (0.1-150 kPa) through a pipette, while the pipette-sample separation is kept constant above the cell surface using ion conductance based distance feedback. This prevents any surface contact, or contamination of the pipette, allowing repeated measurements. We show that we can probe the local mechanical properties of living cells using increasing pressure, and hence measure the nanomechanical properties of the cell membrane and the underlying cytoskeleton in a variety of cells (erythrocytes, epithelium, cardiomyocytes and neurons). Because the cell surface can first be imaged without pressure, it is possible to relate the mechanical properties to the local cell topography. This method is well suited to probe the nanomechanical properties and mechanosensitivity of living cells.
AU  - Sánchez,D
AU  - Johnson,N
AU  - Li,C
AU  - Novak,P
AU  - Rheinlaender,J
AU  - Zhang,Y
AU  - Anand,U
AU  - Anand,P
AU  - Gorelik,J
AU  - Frolenkov,GI
AU  - Benham,C
AU  - Lab,M
AU  - Ostanin,VP
AU  - Schäffer,TE
AU  - Klenerman,D
AU  - Korchev,YE
DO  - 10.1529/biophysj.108.129551
EP  - 27
PY  - 2008///
SN  - 1542-0086
SP  - 3017
TI  - Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette.
T2  - Biophysical journal
UR  - http://dx.doi.org/10.1529/biophysj.108.129551
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2527257&tool=pmcentrez&rendertype=abstract
VL  - 95
ER  -
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