Imperial College London

ProfessorUtaGriesenbach

Faculty of MedicineNational Heart & Lung Institute

Professor of Molecular Medicine
 
 
 
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Contact

 

+44 (0)20 7594 7927u.griesenbach

 
 
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Assistant

 

Miss Samia Soussi +44 (0)20 7594 7980

 
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Location

 

Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Publication Type
Year
to

256 results found

Alton E, Lund-Palau H, Juarez-Molina C, Meng C, Bhargava A, Pilou A, Aziz K, Clarke N, Atsumi N, Ashek A, Wilson M, Takata M, Padley S, Gill D, Hyde S, Morgan C, Griesenbach Uet al., 2022, Correction of a chronic pulmonary disease through lentiviral vector-mediated protein expression, Molecular Therapy - Methods and Clinical Development, Vol: 25, Pages: 382-391, ISSN: 2329-0501

We have developed a novel lentiviral vector, pseudotyped with the F and HN proteins from Sendai virus (rSIV.F/HN), which produces long-lasting, high efficiency transduction of the respiratory epithelium. Here, we addressed whether this platform technology can secrete sufficient levels of a therapeutic protein into the lung to ameliorate a fatal pulmonary disease, as an exemplar of its translational capability. Pulmonary Alveolar Proteinosis (PAP) results from alveolar GM-CSF insufficiency, resulting in abnormal surfactant homeostasis and consequent ventilatory problems. Lungs of GM-CSF knockout mice were transduced with a single dose of rSIV.F/HN expressing murine (m)GM-CSF (1e5-92e7 TU/mouse); mGM-CSF expression was dose-related and persisted for at least 11 months. PAP disease biomarkers were rapidly and persistently corrected, but we noted a narrow toxicity/efficacy window. rSIV.F/HN may be a useful platform technology to deliver therapeutic proteins for lung diseases requiring long-lasting and stable expression of secreted proteins.

Journal article

Clarke NK, Sergijenko A, Pineault K, Sinadinos A, Meng C, Griesenbach U, Alton EWFWet al., 2022, Ex Vivo Transduced Macrophages Engraft in the Lung Following Transplantation and Produce Therapeutic Levels of Secreted Proteins, American Society of Cell and Gene Therapy, Publisher: CELL PRESS, Pages: 405-405, ISSN: 1525-0016

Conference paper

Bell RV, Clarke NK, Isalan M, Alton EWFW, Griesenbach Uet al., 2022, Regulated Expression of Lentiviral Vectors Following Administration of an Inducing Molecule, Publisher: CELL PRESS, Pages: 419-419, ISSN: 1525-0016

Conference paper

Hickmott JW, Prasertsuk P, Bell RV, Chan M, Griesenbach U, Alton EWFWet al., 2022, Heterologous Signal Peptides Enhance Protein Secretion for Respiratory Gene Therapy, Publisher: CELL PRESS, Pages: 417-418, ISSN: 1525-0016

Conference paper

Bell R, Clarke NK, Isalan M, Alton EWFW, Griesenbach Uet al., 2022, Regulated Expression of LentiviralVectors Following Administration of anInducing Molecule, 2022 ASGCT Annual Meeting, Publisher: Cell Press, ISSN: 1525-0016

Conference paper

Sergijenko A, Pineault KM, Moiseenko A, Gill DR, Hyde SC, Kreuz S, Alton EWFW, Griesenbach Uet al., 2022, Development of Molecular Assays to Determine Lentiviral Vector-Mediated Transduction Efficiency in Airway Epithelial Cells, Publisher: CELL PRESS, Pages: 419-419, ISSN: 1525-0016

Conference paper

Clarke N, Sinadinos A, Meng C, Alton E, Griesenbach Uet al., 2021, Ex vivo transduced macrophages produce therapeutic levels of secreted protein when transplanted to the lung, ESGCT Collaborative Virtual Congress, Publisher: Mary Ann Liebert, ISSN: 1043-0342

Conference paper

Bell R, Faulkner N, Griesenbach U, Alton EWFWet al., 2021, Intravenous administration of F/HN pseudotyped lentiviral vector, ESGCT Collaborative Virtual Congress, Publisher: Mary Ann Liebert, ISSN: 1043-0342

Conference paper

Bell RV, Isalan M, Alton EWFW, Griesenbach Uet al., 2021, Regulation of lentivirusā€mediated expression in a human airway model, ESGCT Collaborative Virtual Congress, Publisher: Mary Ann Liebert, ISSN: 1043-0342

Conference paper

Griesenbach U, Yanez-Munoz RJ, 2021, The British Society for Gene and Cell Therapy, HUMAN GENE THERAPY, ISSN: 1043-0342

Journal article

, 2021, ESGCTCollaborative Virtual Congress19–22 October 2021Abstracts, Human Gene Therapy, Vol: 32, Pages: A1-A152, ISSN: 1043-0342

Journal article

Moiseenko A, Pineault K, Sergijenko A, Alton EWFW, Boyd AC, Davies JC, Gill DR, Griesenbach U, Hyde SC, McLachlan G, Hobbie S, Schuler M, Maier U, Thomas MU, Mennerich D, Kreuz Set al., 2021, Functional characterisation of an engineered next generation lentivirus vector for the treatment of cystic fibrosis, Publisher: MARY ANN LIEBERT, INC, Pages: A39-A39, ISSN: 1043-0342

Conference paper

Juarez-Molina CI, Lund-Palau H, Meng C, Gill D, Hyde S, Alton E, Griesenbach Uet al., 2021, Lentiviral vector/GM-CSF Gene Therapy for Autoimmune Pulmonary Alveolar Proteinosis, Publisher: MARY ANN LIEBERT, INC, Pages: A38-A38, ISSN: 1043-0342

Conference paper

Hickmott JW, Prasertsuk P, Bell RV, Chan M, Griesenbach U, Alton EWFWet al., 2021, Modifying signal peptides for respiratory gene therapy with secreted proteins, Publisher: MARY ANN LIEBERT, INC, Pages: A39-A40, ISSN: 1043-0342

Conference paper

Hickmott JW, Sinadinos AJ, Sergijenko A, Saleh AD, Nafchi NAM, Gamlen T, Gill DR, Hyde SC, Alton EWFW, Griesenbach Uet al., 2021, Single cell tools for measuring mRNA and protein expression for gene therapy, Publisher: MARY ANN LIEBERT, INC, Pages: A39-A39, ISSN: 1043-0342

Conference paper

Faulkner N, Alton EW, Griesenbach U, Kassiotis Get al., 2021, Reducing anti-vector humoral immunity through modulation of surface glycoprotein density, Publisher: MARY ANN LIEBERT, INC, Pages: A108-A109, ISSN: 1043-0342

Conference paper

Hickmott JW, Alton EWFW, Griesenbach U, Prasertsuk Pet al., 2021, Signal peptides

Patent

Hickmott JW, Prasertsuk P, Bell RV, Chan M, Griesenbach U, Alton EWFWet al., 2021, Towards Enhancing Signal Peptides for Respiratory Gene Therapy with Secreted Proteins, American Society of Gene and Cell Therapy, Publisher: CELL PRESS, Pages: 139-139, ISSN: 1525-0016

Conference paper

Sergijenko A, Moiseenko A, Pineault K, Nafchi NAM, Chan M, Gamlen T, Gill DR, Hyde SC, Kreuz S, Griesenbach U, Alton EWFWet al., 2021, Assessment of the Air-Liquid Interface Culture Model as a Tool to Validate Efficacy of a rSIV.F/HN-CFTR Vector, Publisher: CELL PRESS, Pages: 260-260, ISSN: 1525-0016

Conference paper

Sinadinos A, Pineault K, Saleh A, Griesenbach U, Alton EWFWet al., 2021, Computational Tools for Airway-Image and Multidimensional-Data Analysis, Publisher: CELL PRESS, Pages: 257-257, ISSN: 1525-0016

Conference paper

Pineault KM, Meng C, Alton EWFW, Griesenbach Uet al., 2021, Biosafety Risk Assessment: Biodistribution and Environmental Shedding of Topically Administered Lentiviral Vector to the Murine Airway, Publisher: CELL PRESS, Pages: 259-260, ISSN: 1525-0016

Conference paper

Clarke NK, Sinadinos A, Meng C, Alton EWFW, Griesenbach Uet al., 2021, Production of Recombinant Protein after Transplantation of Ex Vivo Transduced Macrophages, Publisher: CELL PRESS, Pages: 378-378, ISSN: 1525-0016

Conference paper

Bell RV, Faulkner N, Alton EWFW, Griesenbach Uet al., 2021, Assessment of F/HN Pseudotyped Lentiviral Vector Following Intravenous Delivery to Mice, 24th Annual Meeting of the American Society of Gene & Cell Therapy, Publisher: CELL PRESS, Pages: 138-139, ISSN: 1525-0016

Conference paper

Sinadinos A, Sergijenko A, Meng C, Gamlin T, Hyde S, Gill DR, Griesenbach U, Alton EWFWet al., 2021, Development of Lentiviral Vector Respiratory Tract Dosing Methods in Anticipation of Rodent GLP Toxicology Assessment, Publisher: CELL PRESS, Pages: 220-221, ISSN: 1525-0016

Conference paper

Juarez-Molina CI, Lund-Palau H, Meng C, Gill D, Hyde S, Alton E, Griesenbach Uet al., 2021, Gene Therapy for Autoimmune Pulmonary Alveolar Proteinosis, Publisher: CELL PRESS, Pages: 256-257, ISSN: 1525-0016

Conference paper

Sinadinos AJ, Sergijenko A, Saleh AD, Nafchi NAM, Hickmott JW, Gamlen T, Gill DR, Hyde SC, Alton EWFW, Griesenbach Uet al., 2021, QUANTIFICATION OF MRNA AND PROTEIN FROM SINGLE CELLS FOR CYSTIC FIBROSIS GENE THERAPY, Publisher: BMJ PUBLISHING GROUP, Pages: A50-A50, ISSN: 0040-6376

Conference paper

Sergijenko A, Moiseenko A, Pineault K, Nafchi NAM, Chan M, Gamlen T, Gill DR, Hyde SC, Kreuz S, Griesenbach U, Alton EWFWet al., 2021, LOW LEVELS OF LENTIVIRUS-MEDIATED CFTR GENE TRANSFER ARE SUFFICIENT TO GENERATE ION TRANSPORT CORRECTION IN AIR-LIQUID INTERFACE CULTURES FROM CYSTIC FIBROSIS PATIENTS, Publisher: BMJ PUBLISHING GROUP, Pages: A42-A42, ISSN: 0040-6376

Conference paper

Alton EWFW, Boyd AC, Davies JC, Gill DR, Griesenbach U, Harman TE, Hyde SC, McLachlan Get al., 2021, TOWARDS A FIRST-IN-HUMAN TRIAL WITH A PSEUDOTYPED LENTIVIRUS, Publisher: BMJ PUBLISHING GROUP, Pages: A42-A42, ISSN: 0040-6376

Conference paper

Sinadinos A, Sergijenko A, Meng C, Gamlen T, Hyde S, Gill DR, Griesenbach U, Alton EWFWet al., 2021, DEVELOPMENT OF PROTOCOLS FOR MOUSE GLP-TOXICOLOGY STUDIES, Publisher: BMJ PUBLISHING GROUP, Pages: A32-A32, ISSN: 0040-6376

Conference paper

Buning H, Baker AH, Griesenbach U, Flotte TR, Thrasher AJet al., 2021, Gene and Cell Therapy for Inherited and Acquired Immune Deficiency, HUMAN GENE THERAPY, Vol: 32, Pages: 1-3, ISSN: 1043-0342

Journal article

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