Imperial College London
Alternate

ProfessorUtaGriesenbach

Faculty of Medicine, National Heart & Lung Institute

Professor of Molecular Medicine
 
 
 
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Contact

 

+44 (0)20 7594 7927u.griesenbach

 
 
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Assistant

 

Miss Samia Soussi +44 (0)20 7594 7980

 
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Location

 

Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Leoni:2015:10.2174/1566523215666151016123625#sthash.PBqHlKJC.dpuf,
author = {Leoni, G and Wasowicz, MY and Chan, M and Meng, C and Farley, R and Brody, SL and Inoue, M and Hasegawa, M and Alton, EW and Griesenbach, U},
doi = {10.2174/1566523215666151016123625#sthash.PBqHlKJC.dpuf},
journal = {Current Gene Therapy},
pages = {581--590},
title = {Ex Vivo and In Vivo Lentivirus-Mediated Transduction of Airway Epithelial Progenitor Cells.},
url = {http://dx.doi.org/10.2174/1566523215666151016123625#sthash.PBqHlKJC.dpuf},
volume = {15},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A key challenge in pulmonary gene therapy for cystic fibrosis is to provide long-term correction of the genetic defect. This may be achievable by targeting airway epithelial stem/progenitor cells with an integrating vector. Here, we evaluated the ability of a lentiviral vector, derived from the simian immunodeficiency virus and pseudotyped with F and HN envelope proteins from Sendai virus, to transduce progenitor basal cells of the mouse nasal airways. We first transduced basal cell-enriched cultures ex vivo and confirmed efficient transduction of cytokeratin-5 positive cells. We next asked whether progenitor cells could be transduced in vivo. We evaluated the transduction efficiency in mice pretreated by intranasal administration of polidocanol to expose the progenitor cell layer. Compared to control mice, polidocanol treated mice demonstrated a significant increase in the number of transduced basal cells at 3 and 14 days post vector administration. At 14 days, the epithelium of treated mice contained clusters (4 to 8 adjacent cells) of well differentiated ciliated, as well as basal cells suggesting a clonal expansion. These results indicate that our lentiviral vector can transduce progenitor basal cells in vivo, although transduction required denudation of the surface epithelium prior to vector administration.
AU  - Leoni,G
AU  - Wasowicz,MY
AU  - Chan,M
AU  - Meng,C
AU  - Farley,R
AU  - Brody,SL
AU  - Inoue,M
AU  - Hasegawa,M
AU  - Alton,EW
AU  - Griesenbach,U
DO  - 10.2174/1566523215666151016123625#sthash.PBqHlKJC.dpuf
EP  - 590
PY  - 2015///
SN  - 1875-5631
SP  - 581
TI  - Ex Vivo and In Vivo Lentivirus-Mediated Transduction of Airway Epithelial Progenitor Cells.
T2  - Current Gene Therapy
UR  - http://dx.doi.org/10.2174/1566523215666151016123625#sthash.PBqHlKJC.dpuf
UR  - http://hdl.handle.net/10044/1/27794
VL  - 15
ER  -
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