Imperial College London
Alternate

Dr Umang Shah

Faculty of Engineering, Department of Chemical Engineering

Principal Teaching Fellow
 
 
 
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Contact

 

+44 (0)20 7594 6604u.shah09 Website

 
 
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Location

 

ACEX 210ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Shah:2017:10.1016/j.jcrysgro.2016.09.029,
author = {Shah, UV and Jahn, NH and Huang, S and Yang, Z and Williams, DR and Heng, JYY},
doi = {10.1016/j.jcrysgro.2016.09.029},
journal = {Journal of Crystal Growth},
pages = {42--47},
title = {Crystallisation via novel 3D nanotemplates as a tool for protein purification and bio-separation},
url = {http://dx.doi.org/10.1016/j.jcrysgro.2016.09.029},
volume = {469},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - This study reports an experimental validation of the surface preferential nucleation of proteins on the basis of a relationship between nucleant pore diameter and protein hydrodynamic diameter. The validated correlation was employed for the selection of nucleant pore diameter to crystallise a target protein from binary, equivolume protein mixture. We report proof-of-concept preliminary experimental evidence for the rational approach for crystallisation of a target protein from a binary protein mixture on the surface of 3D nanotemplates with controlled surface porosity and narrow pore-size distribution selected on the basis of a relationship between the nucleant pore diameter and protein hydrodynamic diameter. The outcome of this study opens up an exciting opportunity for exploring protein crystallisation as a potential route for protein purification and bio-separation in both technical and pharmaceutical applications.
AU  - Shah,UV
AU  - Jahn,NH
AU  - Huang,S
AU  - Yang,Z
AU  - Williams,DR
AU  - Heng,JYY
DO  - 10.1016/j.jcrysgro.2016.09.029
EP  - 47
PY  - 2017///
SN  - 0022-0248
SP  - 42
TI  - Crystallisation via novel 3D nanotemplates as a tool for protein purification and bio-separation
T2  - Journal of Crystal Growth
UR  - http://dx.doi.org/10.1016/j.jcrysgro.2016.09.029
UR  - https://www.sciencedirect.com/science/article/pii/S0022024816305322
UR  - http://hdl.handle.net/10044/1/43556
VL  - 469
ER  -
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