Imperial College London
Alternate

DrVeroniqueAzuara

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Reader in Stem Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 1915v.azuara

 
 
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Location

 

1009Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Santos:2010:10.1186/1756-8935-3-1,
author = {Santos, J and Pereira, CF and Di-Gregorio, A and Spruce, T and Alder, O and Rodriguez, T and Azuara, V and Merkenschlager, M and Fisher, AG},
doi = {10.1186/1756-8935-3-1},
journal = {Epigenetics & Chromatin},
title = {Differences in the epigenetic and reprogramming properties of pluripotent and extra-embryonic stem cells implicate chromatin remodelling as an important early event in the developing mouse embryo},
url = {http://dx.doi.org/10.1186/1756-8935-3-1},
volume = {3},
year = {2010}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundDuring early mouse development, two extra-embryonic lineages form alongside the future embryo: the trophectoderm (TE) and the primitive endoderm (PrE). Epigenetic changes known to take place during these early stages include changes in DNA methylation and modified histones, as well as dynamic changes in gene expression.ResultsIn order to understand the role and extent of chromatin-based changes for lineage commitment within the embryo, we examined the epigenetic profiles of mouse embryonic stem (ES), trophectoderm stem (TS) and extra-embryonic endoderm (XEN) stem cell lines that were derived from the inner cell mass (ICM), TE and PrE, respectively. As an initial indicator of the chromatin state, we assessed the replication timing of a cohort of genes in each cell type, based on data that expressed genes and acetylated chromatin domains, generally, replicate early in S-phase, whereas some silent genes, hypoacetylated or condensed chromatin tend to replicate later. We found that many lineage-specific genes replicate early in ES, TS and XEN cells, which was consistent with a broadly 'accessible' chromatin that was reported previously for multiple ES cell lines. Close inspection of these profiles revealed differences between ES, TS and XEN cells that were consistent with their differing lineage affiliations and developmental potential. A comparative analysis of modified histones at the promoters of individual genes showed that in TS and ES cells many lineage-specific regulator genes are co-marked with modifications associated with active (H4ac, H3K4me2, H3K9ac) and repressive (H3K27me3) chromatin. However, in XEN cells several of these genes were marked solely by repressive modifications (such as H3K27me3, H4K20me3). Consistent with TS and XEN having a restricted developmental potential, we show that these cells selectively reprogramme somatic cells to induce the de novo expression of genes associated with extraembryonic differentiation.ConclusionsThese data p
AU  - Santos,J
AU  - Pereira,CF
AU  - Di-Gregorio,A
AU  - Spruce,T
AU  - Alder,O
AU  - Rodriguez,T
AU  - Azuara,V
AU  - Merkenschlager,M
AU  - Fisher,AG
DO  - 10.1186/1756-8935-3-1
PY  - 2010///
SN  - 1756-8935
TI  - Differences in the epigenetic and reprogramming properties of pluripotent and extra-embryonic stem cells implicate chromatin remodelling as an important early event in the developing mouse embryo
T2  - Epigenetics & Chromatin
UR  - http://dx.doi.org/10.1186/1756-8935-3-1
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000283772100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/71588
VL  - 3
ER  -
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