Imperial College London
Dr Vassiliki Koufopanou

DrVassilikiKoufopanou

Faculty of Natural Sciences, Department of Life Sciences (Silwood Park)

Research Fellow in Evolutionary Genetics
 
 
 
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Contact

 

+44 (0)20 7594 2381v.koufopanou

 
 
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Location

 

W3.2Silwood ParkSilwood Park

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Summary

 

Publications

Citation

BibTex format

@article{Koufopanou:2015:gbe/evv112,
author = {Koufopanou, V and Lomas, S and Tsai, IJ and Burt, A},
doi = {gbe/evv112},
journal = {Genome Biology and Evolution},
pages = {1887--1895},
title = {Estimating the fitness effects of new mutations in the wild yeast Saccharomyces paradoxus},
url = {http://dx.doi.org/10.1093/gbe/evv112},
volume = {7},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The nature of selection acting on a population is in large measure determined by the distribution of fitness effects of new mutations. In this study, we use DNA sequences from four closely related clades of Saccharomyces paradoxus and Saccharomyces cerevisiae to identify and polarize new mutations and estimate their fitness effects. By progressively restricting the analyses to narrower categories of sites, we further seek to characterize sites with predictable mutational effects, that is, unconditionally deleterious, neutral or beneficial. Consistent with previous studies on S. paradoxus, we have failed to find evidence for mutations with beneficial effects, even in regions that were divergent in two outgroup clades, perhaps a consequence of the relatively unchallenged, predominantly asexual and highly inbred lifestyle of this species. On the other hand, there is abundant evidence of deleterious mutations, varying in severity of effect from strongly deleterious to very mild, particularly in regions conserved in the outgroup taxa, indicating a history of persistent purifying selection. Narrowing the analysis down to individual amino acids reduces further the range of effects: for example, mutations changing cysteine are predicted to be nearly always strongly deleterious, whereas those changing arginine, serine, and tyrosine are expected to be nearly neutral. The proportion of mutations with deleterious effects for a particular amino acid is correlated with long-term stasis of that amino acid among highly divergent sequences from a variety of organisms, showing that functionality of sites tends to persist through the diversification of clades and that our findings are also relevant to longer evolutionary times and other taxa.
AU  - Koufopanou,V
AU  - Lomas,S
AU  - Tsai,IJ
AU  - Burt,A
DO  - gbe/evv112
EP  - 1895
PY  - 2015///
SN  - 1759-6653
SP  - 1887
TI  - Estimating the fitness effects of new mutations in the wild yeast Saccharomyces paradoxus
T2  - Genome Biology and Evolution
UR  - http://dx.doi.org/10.1093/gbe/evv112
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000358800500004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/44910
VL  - 7
ER  -
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