Imperial College London
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DrVictoriaMale

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Reproductive Immunology
 
 
 
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v.male

 
 
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Location

 

Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gómez:2020:10.1126/scisignal.aax4585,
author = {Gómez, V and Eykyn, TR and Mustapha, R and Flores-Borja, F and Male, V and Barber, PR and Patsialou, A and Green, R and Panagaki, F and Li, CW and Fruhwirth, GO and Ros, S and Brindle, KM and Ng, T},
doi = {10.1126/scisignal.aax4585},
journal = {Science Signaling},
pages = {1--13},
title = {Breast cancer–associated macrophages promote tumorigenesis by suppressing succinate dehydrogenase in tumor cells},
url = {http://dx.doi.org/10.1126/scisignal.aax4585},
volume = {13},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Tumor-associated macrophages (TAMs) can exist in pro- and anti-inflammatory states. Anti-inflammatory TAMs (also referred to as M2-polarized) generally suppress antitumor immune responses and enhance the metastatic progression of cancer. To explore the mechanisms behind this phenomenon, we isolated macrophages from mice and humans, polarized them ex vivo, and examined their functional interaction with breast cancer cells in culture and in mice. We found that anti-inflammatory TAMs promoted a metabolic state in breast cancer cells that supported various protumorigenic phenotypes. Anti-inflammatory TAMs secreted the cytokine TGF-β that, upon engagement of its receptors in breast cancer cells, suppressed the abundance of the transcription factor STAT1 and, consequently, decreased that of the metabolic enzyme succinate dehydrogenase (SDH) in the tumor cells. The decrease in SDH levels in tumor cells resulted in an accumulation of succinate, which enhanced the stability of the transcription factor HIF1α and reprogrammed cell metabolism to a glycolytic state. TAM depletion-repletion experiments in a 4T1 mouse model additionally revealed that anti-inflammatory macrophages promoted HIF-associated vascularization and expression of the immunosuppressive protein PD-L1 in tumors. The findings suggest that anti-inflammatory TAMs promote tumor-associated angiogenesis and immunosuppression by altering metabolism in breast cancer cells.
AU  - Gómez,V
AU  - Eykyn,TR
AU  - Mustapha,R
AU  - Flores-Borja,F
AU  - Male,V
AU  - Barber,PR
AU  - Patsialou,A
AU  - Green,R
AU  - Panagaki,F
AU  - Li,CW
AU  - Fruhwirth,GO
AU  - Ros,S
AU  - Brindle,KM
AU  - Ng,T
DO  - 10.1126/scisignal.aax4585
EP  - 13
PY  - 2020///
SN  - 1945-0877
SP  - 1
TI  - Breast cancer–associated macrophages promote tumorigenesis by suppressing succinate dehydrogenase in tumor cells
T2  - Science Signaling
UR  - http://dx.doi.org/10.1126/scisignal.aax4585
UR  - https://stke.sciencemag.org/content/13/652/eaax4585
UR  - http://hdl.handle.net/10044/1/83180
VL  - 13
ER  -
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