Imperial College London
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DrVikashReebye

Faculty of Medicine, Department of Surgery & Cancer

Research Fellow in Gene Activation
 
 
 
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Contact

 

+44 (0)20 3313 2047v.reebye

 
 
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Location

 

B BlockHammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mintz:2014:10.1038/mt.2013.194,
author = {Mintz, PJ and Huang, K-W and Reebye, V and Nteliopoulos, G and Lai, H-S and Saetrom, P and Kasahara, N and Jensen, S and Pai, M and Gordon, MYA and Marley, SB and Behan, R and Spalding, DR and Haoudi, A and Emara, MM and Nicholls, J and Rossi, JJ and Habib, NA},
doi = {10.1038/mt.2013.194},
journal = {Molecular Therapy},
pages = {149--159},
title = {Exploiting human CD34(+) stem cell-conditioned medium for tissue repair},
url = {http://dx.doi.org/10.1038/mt.2013.194},
volume = {22},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34+ cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble factors in medium conditioned by adherent human CD34+ cells. Thirty-two abundantly secreted factors have been identified, all of which are associated with cell proliferation, survival, tissue repair, and wound healing. The cultured CD34+ cells expressed known stem cell genes such as Nanog, Oct4, Sox2, c-kit, and HoxB4. The conditioned medium containing the secreted factors prevented cell death in liver cells exposed to liver toxin in vitro via inhibition of the caspase-3 signaling pathway. More importantly, in vivo studies using animal models of liver damage demonstrated that injection of the conditioned medium could repair damaged liver tissue (significant reduction in the necroinflammatory activity), as well as enable the animals to survive. Thus, we demonstrate that medium conditioned by human CD34+ cells has the potential for therapeutic repair of damaged tissue in vivo.
AU  - Mintz,PJ
AU  - Huang,K-W
AU  - Reebye,V
AU  - Nteliopoulos,G
AU  - Lai,H-S
AU  - Saetrom,P
AU  - Kasahara,N
AU  - Jensen,S
AU  - Pai,M
AU  - Gordon,MYA
AU  - Marley,SB
AU  - Behan,R
AU  - Spalding,DR
AU  - Haoudi,A
AU  - Emara,MM
AU  - Nicholls,J
AU  - Rossi,JJ
AU  - Habib,NA
DO  - 10.1038/mt.2013.194
EP  - 159
PY  - 2014///
SN  - 1525-0016
SP  - 149
TI  - Exploiting human CD34(+) stem cell-conditioned medium for tissue repair
T2  - Molecular Therapy
UR  - http://dx.doi.org/10.1038/mt.2013.194
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000329213500018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S1525001616311297?via%3Dihub
UR  - http://hdl.handle.net/10044/1/73278
VL  - 22
ER  -
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