Imperial College London
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DrVictoriaSalem

Faculty of Engineering, Department of Bioengineering

Clinical Senior Lecturer in Diabetes and Endocrinology
 
 
 
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v.salem

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Akalestou:2020:10.1101/2020.05.05.078725,
author = {Akalestou, E and Suba, K and Lopez-Noriega, L and Georgiadou, E and Chabosseau, P and Leclerc, I and Salem, V and Rutter, GA},
doi = {10.1101/2020.05.05.078725},
title = {Intravital imaging of islet Ca<sup>2+</sup> dynamics reveals enhanced β cell connectivity after bariatric surgery in mice},
url = {http://dx.doi.org/10.1101/2020.05.05.078725},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>Bariatric surgery improves both insulin sensitivity and secretion in type 2 diabetes. However, these changes are difficult to monitor directly and independently. In particular, the degree and the time course over which surgery impacts β cell function, versus mass, have been difficult to establish. In this study, we investigated the effect of bariatric surgery on β cell function <jats:italic>in vivo</jats:italic> by imaging Ca<jats:sup>2+</jats:sup> dynamics prospectively and at the single cell level in islets engrafted into the anterior eye chamber. Islets expressing GCaMP6f selectively in the β cell were transplanted into obese male hyperglycaemic mice that were then subjected to either vertical sleeve gastrectomy (VSG) or sham surgery. Imaged <jats:italic>in vivo</jats:italic> in the eye, VSG improved coordinated Ca<jats:sup>2+</jats:sup> activity, with 90% of islets observed exhibiting enhanced Ca<jats:sup>2+</jats:sup> wave activity ten weeks post-surgery, while islet wave activity in sham animals fell to zero discernible coordinated islet Ca<jats:sup>2+</jats:sup> activity at the same time point. Correspondingly, VSG mice displayed significantly improved glucose tolerance and insulin secretion. Circulating fasting levels of GLP-1 were also increased after surgery, potentially contributing to improved β cell performance. We thus demonstrate that bariatric surgery leads to time-dependent increases in individual β cell function and intra-islet connectivity, together driving increased insulin secretion and diabetes remission, in a weight-loss independent fashion.</jats:p><jats:sec><jats:title>Significance Statement</jats:title><jats:p>Used widely to treat obesity, bariatric surgery also relieves the symptoms of type 2 diabetes. The mechanisms involved in diabetes remission are still
AU  - Akalestou,E
AU  - Suba,K
AU  - Lopez-Noriega,L
AU  - Georgiadou,E
AU  - Chabosseau,P
AU  - Leclerc,I
AU  - Salem,V
AU  - Rutter,GA
DO  - 10.1101/2020.05.05.078725
PY  - 2020///
TI  - Intravital imaging of islet Ca<sup>2+</sup> dynamics reveals enhanced β cell connectivity after bariatric surgery in mice
UR  - http://dx.doi.org/10.1101/2020.05.05.078725
ER  -
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