Imperial College London
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ProfessorVincentSavolainen

Faculty of Natural Sciences, Department of Life Sciences (Silwood Park)

Professor of Organismic Biology
 
 
 
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Contact

 

v.savolainen CV

 
 
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Assistant

 

Ms Elisabeth Ahlstrom +44 (0)20 7594 2207

 
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Location

 

N.1-17MunroSilwood Park

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Summary

 

Publications

Citation

BibTex format

@article{Osborne:2023:10.1111/nph.18645,
author = {Osborne, OG and Dobreva, MP and Papadopulos, AST and de, Moura MSB and Brunello, AT and de, Queiroz LP and Pennington, RT and Lloyd, J and Savolainen, V},
doi = {10.1111/nph.18645},
journal = {New Phytologist},
pages = {1305--1317},
title = {Mapping the root systems of individual trees in a natural community using genotyping-by-sequencing},
url = {http://dx.doi.org/10.1111/nph.18645},
volume = {238},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - •The architecture of root systems is an important driver of plant fitness, competition and ecosystem processes. However, the methodological difficulty of mapping roots hampers the study of these processes. Existing approaches to match individual plants to belowground samples are low throughput and species specific. Here, we developed a scalable sequencing-based method to map the root systems of individual trees across multiple species. We successfully applied it to a tropical dry forest community in the Brazilian Caatinga containing 14 species.   • We sequenced all 42 individual shrubs and trees in a 14 × 14 m plot using double-digest restriction site-associated sequencing (ddRADseq). We identified species-specific markers and individual-specific haplotypes from the data. We matched these markers to the ddRADseq data from 100 mixed root samples from across the centre (10 × 10 m) of the plot at four different depths using a newly developed R package.   • We identified individual root samples for all species and all but one individual. There was a strong significant correlation between belowground and aboveground size measurements, and we also detected significant species-level root-depth preference for two species.   • The method is more scalable and less labour intensive than the current techniques and is broadly applicable to ecology, forestry and agricultural biology.
AU  - Osborne,OG
AU  - Dobreva,MP
AU  - Papadopulos,AST
AU  - de,Moura MSB
AU  - Brunello,AT
AU  - de,Queiroz LP
AU  - Pennington,RT
AU  - Lloyd,J
AU  - Savolainen,V
DO  - 10.1111/nph.18645
EP  - 1317
PY  - 2023///
SN  - 0028-646X
SP  - 1305
TI  - Mapping the root systems of individual trees in a natural community using genotyping-by-sequencing
T2  - New Phytologist
UR  - http://dx.doi.org/10.1111/nph.18645
UR  - https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18645
UR  - http://hdl.handle.net/10044/1/101890
VL  - 238
ER  -
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