Publications

BibTex format

@article{Joyce:2012:10.1371/journal.pone.0044582,
author = {Joyce, G and Williams, KJ and Robb, M and Noens, E and Tizzano, B and Shahrezaei, V and Robertson, BD},
doi = {10.1371/journal.pone.0044582},
journal = {PLOS One},
title = {Cell Division Site Placement and Asymmetric Growth in Mycobacteria},
url = {http://dx.doi.org/10.1371/journal.pone.0044582},
volume = {7},
year = {2012}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Mycobacteria are members of the actinomycetes that grow by tip extension and lack apparent homologues of the known cell division regulators found in other rod-shaped bacteria. Previous work using static microscopy on dividing mycobacteria led to the hypothesis that these cells can grow and divide asymmetrically, and at a wide range of sizes, in contrast to the cell growth and division patterns observed in the model rod-shaped organisms. In this study, we test this hypothesis using live-cell time-lapse imaging of dividing Mycobacterium smegmatis labelled with fluorescent PBP1a, to probe peptidoglycan synthesis and label the cell septum. We demonstrate that the new septum is placed accurately at mid-cell, and that the asymmetric division observed is a result of differential growth from the cell tips, with a more than 2-fold difference in growth rate between fast and slow growing poles. We also show that the division site is not selected at a characteristic cell length, suggesting this is not an important cue during the mycobacterial cell cycle.
AU  - Joyce,G
AU  - Williams,KJ
AU  - Robb,M
AU  - Noens,E
AU  - Tizzano,B
AU  - Shahrezaei,V
AU  - Robertson,BD
DO  - 10.1371/journal.pone.0044582
PY  - 2012///
SN  - 1932-6203
TI  - Cell Division Site Placement and Asymmetric Growth in Mycobacteria
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0044582
UR  - http://hdl.handle.net/10044/1/24071
VL  - 7
ER  -

Download

DrVahidShahrezaei

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)