Imperial College London
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Vincenzo De Paola

Faculty of Medicine, Department of Brain Sciences

Reader in Translational Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 2501vincenzo.depaola Website CV

 
 
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Assistant

 

Miss Lydia Lawson +44 (0)20 7594 1264

 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Canty:2020:10.1186/s12915-020-00869-2,
author = {Canty, AJ and Jackson, JS and Huang, L and Trabalza, A and Bass, C and Little, G and Tortora, M and Khan, S and De, Paola V},
doi = {10.1186/s12915-020-00869-2},
journal = {BMC Biology},
title = {In vivo imaging of injured cortical axons reveals a rapid onset form of Wallerian degeneration},
url = {http://dx.doi.org/10.1186/s12915-020-00869-2},
volume = {18},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:sec>                <jats:title>Background</jats:title>                <jats:p>Despite the widespread occurrence of axon and synaptic loss in the injured and diseased nervous system, the cellular and molecular mechanisms of these key degenerative processes remain incompletely understood. Wallerian degeneration (WD) is a tightly regulated form of axon loss after injury, which has been intensively studied in large myelinated fibre tracts of the spinal cord, optic nerve and peripheral nervous system (PNS). Fewer studies, however, have focused on WD in the complex neuronal circuits of the mammalian brain, and these were mainly based on conventional endpoint histological methods. Post-mortem analysis, however, cannot capture the exact sequence of events nor can it evaluate the influence of elaborated arborisation and synaptic architecture on the degeneration process, due to the non-synchronous and variable nature of WD across individual axons.</jats:p>              </jats:sec><jats:sec>                <jats:title>Results</jats:title>                <jats:p>To gain a comprehensive picture of the spatiotemporal dynamics and synaptic mechanisms of WD in the nervous system, we identify the factors that regulate WD within the mouse cerebral cortex. We combined single-axon-resolution multiphoton imaging with laser microsurgery through a cranial window and a fluorescent membrane reporter. Longitudinal imaging of > 150 individually injured excitatory cortical axons revealed a threshold length below which injured axons consistently underwent a rapid-onset form of WD (roWD). roWD started on average 20 times earlier and was executed 3 times slower than WD described in other regions of the nervous system. Cortical axon WD and roWD were dependent on synaptic density, but independent of axon complexity. Finally, pharmacological and genetic manipulations showed t
AU  - Canty,AJ
AU  - Jackson,JS
AU  - Huang,L
AU  - Trabalza,A
AU  - Bass,C
AU  - Little,G
AU  - Tortora,M
AU  - Khan,S
AU  - De,Paola V
DO  - 10.1186/s12915-020-00869-2
PY  - 2020///
TI  - In vivo imaging of injured cortical axons reveals a rapid onset form of Wallerian degeneration
T2  - BMC Biology
UR  - http://dx.doi.org/10.1186/s12915-020-00869-2
VL  - 18
ER  -
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