Imperial College London
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Vincenzo De Paola

Faculty of Medicine, Department of Brain Sciences

Reader in Translational Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 2501vincenzo.depaola Website CV

 
 
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Assistant

 

Miss Lydia Lawson +44 (0)20 7594 1264

 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Calderon:2022:10.7554/eLife.76539,
author = {Calderon, L and Weiss, FD and Beagan, JA and Oliveira, MS and Georgieva, R and Wang, Y-F and Carroll, TS and Dharmalingam, G and Gong, W and Tossell, K and de, Paola V and Whilding, C and Ungless, MA and Fisher, AG and Phillips-Cremins, JE and Merkenschlager, M},
doi = {10.7554/eLife.76539},
journal = {eLife},
title = {Cohesin-dependence of neuronal gene expression relates to chromatin loop length},
url = {http://dx.doi.org/10.7554/eLife.76539},
volume = {11},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cohesin and CTCF are major drivers of 3D genome organization, but their role in neurons is still emerging. Here, we show a prominent role for cohesin in the expression of genes that facilitate neuronal maturation and homeostasis. Unexpectedly, we observed two major classes of activity-regulated genes with distinct reliance on cohesin in mouse primary cortical neurons. Immediate early genes (IEGs) remained fully inducible by KCl and BDNF, and short-range enhancer-promoter contacts at the IEGs Fos formed robustly in the absence of cohesin. In contrast, cohesin was required for full expression of a subset of secondary response genes characterized by long-range chromatin contacts. Cohesin-dependence of constitutive neuronal genes with key functions in synaptic transmission and neurotransmitter signaling also scaled with chromatin loop length. Our data demonstrate that key genes required for the maturation and activation of primary cortical neurons depend on cohesin for their full expression, and that the degree to which these genes rely on cohesin scales with the genomic distance traversed by their chromatin contacts.Editor's
AU  - Calderon,L
AU  - Weiss,FD
AU  - Beagan,JA
AU  - Oliveira,MS
AU  - Georgieva,R
AU  - Wang,Y-F
AU  - Carroll,TS
AU  - Dharmalingam,G
AU  - Gong,W
AU  - Tossell,K
AU  - de,Paola V
AU  - Whilding,C
AU  - Ungless,MA
AU  - Fisher,AG
AU  - Phillips-Cremins,JE
AU  - Merkenschlager,M
DO  - 10.7554/eLife.76539
PY  - 2022///
SN  - 2050-084X
TI  - Cohesin-dependence of neuronal gene expression relates to chromatin loop length
T2  - eLife
UR  - http://dx.doi.org/10.7554/eLife.76539
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000796608400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://elifesciences.org/articles/76539
UR  - http://hdl.handle.net/10044/1/97645
VL  - 11
ER  -
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