Imperial College London
Alternate

DrWengangChai

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2596w.chai

 
 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chai:2021:glycob/cwab037,
author = {Chai, W and Li, C and Palma, A and Zhang, P and Zhang, Y and Gao, C and Silva, L and Li, Z and Trovao, F and Weishaupt, M and Seeberger, P and Likhosherstov, L and Piskarev, V and Yu, J and Westerlind, U},
doi = {glycob/cwab037},
journal = {Glycobiology},
pages = {931--946},
title = {Noncovalent microarrays from synthetic amino-terminating glycans: Implications in expanding glycan microarray diversity and platform comparison},
url = {http://dx.doi.org/10.1093/glycob/cwab037},
volume = {31},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Glycan microarrays have played important roles in detection and specificity assignment of glycan recognition by proteins. However, the size and diversity of glycan libraries in current microarray systems are small compared to estimated glycomes, and these may lead to missed detection or incomplete assignment. For microarray construction, covalent and noncovalent immobilization are the two types of methods used, but a direct comparison of results from the two platforms is required. Here we develop a chemical strategy to prepare lipid-linked probes from both naturally derived aldehyde-terminating and synthetic amino-terminating glycans that addresses the two aspects: expansion of sequence-defined glycan libraries and comparison of the two platforms. We demonstrate the specific recognition by plant and mammalian lectins, carbohydrate-binding modules and antibodies and the overall similarities from the two platforms. Our results provide new knowledge on unique glycan-binding specificities for the immune receptor Dectin-1 toward β-glucans and the interaction of rotavirus P[19] adhesive protein with mucin O-glycan cores.
AU  - Chai,W
AU  - Li,C
AU  - Palma,A
AU  - Zhang,P
AU  - Zhang,Y
AU  - Gao,C
AU  - Silva,L
AU  - Li,Z
AU  - Trovao,F
AU  - Weishaupt,M
AU  - Seeberger,P
AU  - Likhosherstov,L
AU  - Piskarev,V
AU  - Yu,J
AU  - Westerlind,U
DO  - glycob/cwab037
EP  - 946
PY  - 2021///
SN  - 0959-6658
SP  - 931
TI  - Noncovalent microarrays from synthetic amino-terminating glycans: Implications in expanding glycan microarray diversity and platform comparison
T2  - Glycobiology
UR  - http://dx.doi.org/10.1093/glycob/cwab037
UR  - https://academic.oup.com/glycob/advance-article/doi/10.1093/glycob/cwab037/6272443
UR  - http://hdl.handle.net/10044/1/89475
VL  - 31
ER  -
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