Imperial College London
Alternate

ProfessorWaljitDhillo

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Endocrinology & Metabolism
 
 
 
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Contact

 

+44 (0)20 7594 3487w.dhillo Website

 
 
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Assistant

 

Ms Suzanne Wheeler +44 (0)20 7594 3487

 
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Location

 

6N6ECommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Izzi-Engbeaya:2020:10.3390/ijms21197394,
author = {Izzi-Engbeaya, C and Abbara, A and Cass, A and Dhillo, W},
doi = {10.3390/ijms21197394},
journal = {International Journal of Molecular Sciences},
title = {Using Aptamers as a Novel Method for Determining GnRH/LH Pulsatility},
url = {http://dx.doi.org/10.3390/ijms21197394},
volume = {21},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Aptamers are a novel technology enabling the continuous measurement of analytes in blood and other body compartments, without the need for repeated sampling and the associated reagent costs of traditional antibody-based methodologies. Aptamers are short single-stranded synthetic RNA or DNA that recognise and bind to specific targets. The conformational changes that can occur upon aptamer–ligand binding are transformed into chemical, fluorescent, colour changes and other readouts. Aptamers have been developed to detect and measure a variety of targets in vitro and in vivo. Gonadotropin-releasing hormone (GnRH) is a pulsatile hypothalamic hormone that is essential for normal fertility but difficult to measure in the peripheral circulation. However, pulsatile GnRH release results in pulsatile luteinizing hormone (LH) release from the pituitary gland. As such, LH pulsatility is the clinical gold standard method to determine GnRH pulsatility in humans. Aptamers have recently been shown to successfully bind to and measure GnRH and LH, and this review will focus on this specific area. However, due to the adaptability of aptamers, and their suitability for incorporation into portable devices, aptamer-based technology is likely to be used more widely in the future.
AU  - Izzi-Engbeaya,C
AU  - Abbara,A
AU  - Cass,A
AU  - Dhillo,W
DO  - 10.3390/ijms21197394
PY  - 2020///
SN  - 1422-0067
TI  - Using Aptamers as a Novel Method for Determining GnRH/LH Pulsatility
T2  - International Journal of Molecular Sciences
UR  - http://dx.doi.org/10.3390/ijms21197394
UR  - https://www.mdpi.com/1422-0067/21/19/7394
UR  - http://hdl.handle.net/10044/1/84671
VL  - 21
ER  -
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