Imperial College London
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DrWafaKhamri

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Teaching Fellow
 
 
 
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Contact

 

w.khamri

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bernsmeier:2018:10.1136/gutjnl-2017-314184,
author = {Bernsmeier, C and Triantafyllou, E and Brenig, R and Lebosse, FJ and Singanayagam, A and Patel, VC and Pop, OT and Khamri, W and Nathwani, R and Tidswell, R and Weston, CJ and Adams, DH and Thursz, MR and Wendon, JA and Antoniades, CG},
doi = {10.1136/gutjnl-2017-314184},
journal = {Gut},
pages = {1155--1167},
title = {CD14<sup>+ </sup>CD15<sup>− </sup>HLA-DR<sup>−</sup> myeloid-derived suppressor cells impair antimicrobial responses in patients with acute-on-chronic liver failure},
url = {http://dx.doi.org/10.1136/gutjnl-2017-314184},
volume = {67},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:sec><jats:title>Objective</jats:title><jats:p>Immune paresis in patients with acute-on-chronic liver failure (ACLF) accounts for infection susceptibility and increased mortality. Immunosuppressive mononuclear CD14<jats:sup>+</jats:sup>HLA-DR<jats:sup>−</jats:sup> myeloid-derived suppressor cells (M-MDSCs) have recently been identified to quell antimicrobial responses in immune-mediated diseases. We sought to delineate the function and derivation of M-MDSC in patients with ACLF, and explore potential targets to augment antimicrobial responses.</jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p>Patients with ACLF (n=41) were compared with healthy subjects (n=25) and patients with cirrhosis (n=22) or acute liver failure (n=30). CD14<jats:sup>+</jats:sup>CD15<jats:sup>−</jats:sup>CD11b<jats:sup>+</jats:sup>HLA-DR<jats:sup>−</jats:sup> cells were identified as per definition of M-MDSC and detailed immunophenotypic analyses were performed. Suppression of T cell activation was assessed by mixed lymphocyte reaction. Assessment of innate immune function included cytokine expression in response to Toll-like receptor (TLR-2, TLR-4 and TLR-9) stimulation and phagocytosis assays using flow cytometry and live cell imaging-based techniques.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Circulating CD14<jats:sup>+</jats:sup>CD15<jats:sup>−</jats:sup>CD11b<jats:sup>+</jats:sup>HLA-DR<jats:sup>−</jats:sup> M-MDSCs were markedly expanded in patients with ACLF (55% of CD14+ cells). M-MDSC displayed immunosuppressive properties, significantly decreasing T cell proliferation (p=0.01), producing less tumour necrosis factor-alpha/interleukin-6 in response to TLR stimulation (all p<0.01), and redu
AU  - Bernsmeier,C
AU  - Triantafyllou,E
AU  - Brenig,R
AU  - Lebosse,FJ
AU  - Singanayagam,A
AU  - Patel,VC
AU  - Pop,OT
AU  - Khamri,W
AU  - Nathwani,R
AU  - Tidswell,R
AU  - Weston,CJ
AU  - Adams,DH
AU  - Thursz,MR
AU  - Wendon,JA
AU  - Antoniades,CG
DO  - 10.1136/gutjnl-2017-314184
EP  - 1167
PY  - 2018///
SN  - 0017-5749
SP  - 1155
TI  - CD14<sup>+ </sup>CD15<sup>− </sup>HLA-DR<sup>−</sup> myeloid-derived suppressor cells impair antimicrobial responses in patients with acute-on-chronic liver failure
T2  - Gut
UR  - http://dx.doi.org/10.1136/gutjnl-2017-314184
UR  - http://hdl.handle.net/10044/1/48341
VL  - 67
ER  -
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