Imperial College London
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DrWing MayKong

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Senior Lecturer in Endocrinology and Ethics
 
 
 
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Contact

 

+44 (0)20 7594 0881w.kong Website

 
 
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Location

 

325Reynolds BuildingCharing Cross Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kong:2003:10.1096/fj.02-0805fje,
author = {Kong, WM and Stanley, S and Gardiner, J and Abbott, C and Murphy, K and Seth, A and Connoley, I and Ghatei, M and Stephens, D and Bloom, S},
doi = {10.1096/fj.02-0805fje},
journal = {The FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
pages = {1688--1690},
title = {A role for arcuate cocaine and amphetamine-regulated transcript in hyperphagia, thermogenesis, and cold adaptation.},
url = {http://dx.doi.org/10.1096/fj.02-0805fje},
volume = {17},
year = {2003}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We have recently shown that injection of the hypothalamic peptide cocaine and amphetamine regulated transcript (CART) into discrete hypothalamic nuclei stimulates food intake. This stimulation was particularly marked in the arcuate nucleus. Here we show that twice daily intra-arcuate injection of 0.2 nmole CART peptide for 7 days was associated with a 60% higher daytime food intake, an 85% higher thermogenic response to the beta3 agonist BRL 35135, and a 60% increase in brown adipose tissue UCP-1 mRNA. In a separate study, using stereotactically targeted gene transfer, a CART transgene was delivered by using polyethylenimine to the arcuate nucleus of adult rats. Food intake was increased significantly during ad libitum feeding and following periods of food withdrawal and food restriction in CART over-expressing animals. CART over-expressing animals lost 12% more weight than controls following a 24-h fast. Brown adipose tissue uncoupling protein-1 (UCP-1) mRNA levels (collected Day 25) were 80% higher in CART over-expressing animals. Finally, by using quantitative in situ hybridization, we found that chronic cold exposure (20 days at 4oC) increased arcuate nucleus CART mRNA by 124%. Together with the orexigenic and thermogenic effects of CART, this finding suggests a role for arcuate nucleus CART in cold adaptation.
AU  - Kong,WM
AU  - Stanley,S
AU  - Gardiner,J
AU  - Abbott,C
AU  - Murphy,K
AU  - Seth,A
AU  - Connoley,I
AU  - Ghatei,M
AU  - Stephens,D
AU  - Bloom,S
DO  - 10.1096/fj.02-0805fje
EP  - 1690
PY  - 2003///
SP  - 1688
TI  - A role for arcuate cocaine and amphetamine-regulated transcript in hyperphagia, thermogenesis, and cold adaptation.
T2  - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
UR  - http://dx.doi.org/10.1096/fj.02-0805fje
VL  - 17
ER  -
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