Publications
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Kim Y, Leung MHY, Kwok W, et al., 2020, Antibiotic resistance gene sharing networks and the effect of dietary nutritional content on the canine and feline gut resistome, Animal Microbiome, Vol: 2, Pages: 1-14, ISSN: 2524-4671
BackgroundAs one of the most densely populated microbial communities on Earth, the gut microbiota serves as an important reservoir of antibiotic resistance genes (ARGs), referred to as the gut resistome. Here, we investigated the association of dietary nutritional content with gut ARG diversity and composition, using publicly available shotgun metagenomic sequence data generated from canine and feline fecal samples. Also, based on network theory, we explored ARG-sharing patterns between gut bacterial genera by identifying the linkage structure between metagenomic assemblies and their functional genes obtained from the same data.ResultsIn both canine and feline gut microbiota, an increase in protein and a reduction in carbohydrate in the diet were associated with increased ARG diversity. ARG diversity of the canine gut microbiota also increased, but less strongly, after a reduction in protein and an increase in carbohydrate in the diet. The association between ARG and taxonomic composition suggests that diet-induced changes in the gut microbiota may be responsible for changes in ARG composition, supporting the links between protein metabolism and antibiotic resistance in gut microbes. In the analysis of the ARG-sharing patterns, 22 ARGs were shared among 46 genera in the canine gut microbiota, and 11 ARGs among 28 genera in the feline gut microbiota. Of these ARGs, the tetracycline resistance gene tet(W) was shared among the largest number of genera, predominantly among Firmicutes genera. Bifidobacterium, a genus extensively used in the fermentation of dairy products and as probiotics, shared tet(W) with a wide variety of other genera. Finally, genera from the same phylum were more likely to share ARGs than with those from different phyla.ConclusionsOur findings show that dietary nutritional content, especially protein content, is associated with the gut resistome and suggest future research to explore the impact of dietary intervention on the development of antibiot
Dunbar D, Kwok W, Graham E, et al., 2019, Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates, JOURNAL OF FELINE MEDICINE AND SURGERY, Vol: 21, Pages: 910-921, ISSN: 1098-612X
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- Citations: 18
Kong F, Kwok W, Pfeiffer D, 2019, Integrating transportation and economic information for the African Swine Fever Virus (ASFV) risk modelling purposes in the Greater Mekong Sub-region (GMS) using online data and grey literature, GeoVet
Kwok W, Kennedy N, Brodbelt D, et al., 2018, Unlocking the potential of veterinary clinical records using a data mining approach, 15th International Symposium of Veterinary Epidemiology and Economics
Kwok W, Mellor KC, 2018, Is topical or systemic antimicrobial therapy more effective for the treatment of feline acne with secondary bacterial infection?, Veterinary Evidence, Vol: 3, Pages: 1-7, ISSN: 2396-9776
PICO questionIn cats with feline acne and secondary bacterial folliculitis or furunculosis, is topical or systemic antimicrobial therapy superior for reducing time to resolution and severity of clinical signs?Clinical bottom lineThere is no sufficient evidence to compare topical versus systemic treatment in feline acne with secondary folliculitis/furunculosis.
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