Imperial College London

Prof. William Wisden F. Med. Sci.

Faculty of Natural SciencesDepartment of Life Sciences

Chair in Molecular Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 9744w.wisden Website CV

 
 
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Location

 

401BSir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Jones:1997,
author = {Jones, A and Korpi, ER and McKernan, RM and Pelz, R and Nusser, Z and Makela, R and Mellor, JR and Pollard, S and Bahn, S and Stephenson, FA and Randall, AD and Sieghart, W and Somogyi, P and Smith, AJ and Wisden, W},
journal = {J Neurosci},
pages = {1350--1362},
title = {Ligand-gated ion channel subunit partnerships: GABA-A receptor alpha6 subunit gene inactivation inhibits delta subunit expression},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9006978},
volume = {17},
year = {1997}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Cerebellar granule cells express six GABA-A receptor subunits abundantly (alpha1, alpha6, beta2, beta3, gamma2, and delta) and assemble various pentameric receptor subtypes with unknown subunit compositions; however, the rules guiding receptor subunit assembly are unclear. Here, removal of intact alpha6 protein from cerebellar granule cells allowed perturbations in other subunit levels to be studied. Exon 8 of the mouse alpha6 subunit gene was disrupted by homologous recombination. In alpha6 -/- granule cells, the delta subunit was selectively degraded as seen by immunoprecipitation, immunocytochemistry, and immunoblot analysis with delta subunit-specific antibodies. The delta subunit mRNA was present at wild-type levels in the mutant granule cells, indicating a post-translational loss of the delta subunit. These results provide genetic evidence for a specific association between the alpha6 and delta subunits. Because in alpha6 -/- neurons the remaining alpha1, beta2/3, and gamma2 subunits cannot rescue the delta subunit, certain potential subunit combinations may not be found in wild-type cells.
AU - Jones,A
AU - Korpi,ER
AU - McKernan,RM
AU - Pelz,R
AU - Nusser,Z
AU - Makela,R
AU - Mellor,JR
AU - Pollard,S
AU - Bahn,S
AU - Stephenson,FA
AU - Randall,AD
AU - Sieghart,W
AU - Somogyi,P
AU - Smith,AJ
AU - Wisden,W
EP - 1362
PY - 1997///
SN - 0270-6474
SP - 1350
TI - Ligand-gated ion channel subunit partnerships: GABA-A receptor alpha6 subunit gene inactivation inhibits delta subunit expression
T2 - J Neurosci
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9006978
VL - 17
ER -