Imperial College London

Prof. William Wisden F. Med. Sci.

Faculty of Natural SciencesDepartment of Life Sciences

Chair in Molecular Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 9744w.wisden Website CV

 
 
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Location

 

401BSir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wisden:1997,
author = {Wisden, W},
journal = {Biochemical Society Transactions},
title = {Ligand-gated ion channel subunit partnerships: GABA<inf>A</inf> receptor α<inf>6</inf> subunit gene inactivation inhibits δ subunit expression},
volume = {25},
year = {1997}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Cerebellar granule cells express six GABAA receptor subunits abundantly (α1, α6, β2, β3, γ2 and δ), and assemble a variety of pentameric receptor subtypes, with unknown subunit compositions. However, the rules guiding receptor subunit assembly are unclear. Removal of intact α6 protein from cerebellar granule cells allowed perturbations in other subunit levels to be studied (Jones et al., 1997). Exon 8 of the mouse α6 -/- subunit gene was disrupted by homologous recombination. In α6 -1- granule cells, the δ subunit was selectively degraded as seen by immunoprecipitation, immunocytochemistry, and immunoblot analysis with δ subunit-specific antibodies. The δ subunit mRNA was present at wild-type levels in the mutant granule cells, indicating a post-translational loss of the δ subunit. These results provide genetic evidence for a specific association between the α6 and δ subunits.
AU - Wisden,W
PY - 1997///
SN - 0300-5127
TI - Ligand-gated ion channel subunit partnerships: GABA<inf>A</inf> receptor α<inf>6</inf> subunit gene inactivation inhibits δ subunit expression
T2 - Biochemical Society Transactions
VL - 25
ER -