Imperial College London
Alternate

DrWeiCui

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2124wei.cui Website

 
 
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Location

 

1010Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sheng:2020:10.1016/j.celrep.2020.107952,
author = {Sheng, X and Lin, Z and Lv, C and Shao, C and Bi, X and Deng, M and Xu, J and Guerrero-Juarez, CF and Li, M and Wu, X and Zhao, R and Yang, X and Li, G and Liu, X and Wang, Q and Nie, Q and Cui, W and Gao, S and Zhang, H and Liu, Z and Cong, Y and Plikus, MV and Lengner, CJ and Andersen, B and Ren, F and Yu, Z},
doi = {10.1016/j.celrep.2020.107952},
journal = {Cell Reports},
pages = {107952--107952},
title = {Cycling Stem Cells Are Radioresistant and Regenerate the Intestine.},
url = {http://dx.doi.org/10.1016/j.celrep.2020.107952},
volume = {32},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A certain number of epithelial cells in intestinal crypts are DNA damage resistant and contribute to regeneration. However, the cellular mechanism underlying intestinal regeneration remains unclear. Using lineage tracing, we show that cells marked by an Msi1 reporter (Msi1+) are right above Lgr5high cells in intestinal crypts and exhibit DNA damage resistance. Single-cell RNA sequencing reveals that the Msi1+ cells are heterogeneous with the majority being intestinal stem cells (ISCs). The DNA damage-resistant subpopulation of Msi1+ cells is characterized by low-to-negative Lgr5 expression and is more rapidly cycling than Lgr5high radiosensitive crypt base columnar stem cells (CBCs). This enables an efficient repopulation of the intestinal epithelium at early stage when Lgr5high cells are not emerging. Furthermore, relative to CBCs, Msi1+ cells preferentially produce Paneth cells during homeostasis and upon radiation repair. Together, we demonstrate that the DNA damage-resistant Msi1+ cells are cycling ISCs that maintain and regenerate the intestinal epithelium.
AU  - Sheng,X
AU  - Lin,Z
AU  - Lv,C
AU  - Shao,C
AU  - Bi,X
AU  - Deng,M
AU  - Xu,J
AU  - Guerrero-Juarez,CF
AU  - Li,M
AU  - Wu,X
AU  - Zhao,R
AU  - Yang,X
AU  - Li,G
AU  - Liu,X
AU  - Wang,Q
AU  - Nie,Q
AU  - Cui,W
AU  - Gao,S
AU  - Zhang,H
AU  - Liu,Z
AU  - Cong,Y
AU  - Plikus,MV
AU  - Lengner,CJ
AU  - Andersen,B
AU  - Ren,F
AU  - Yu,Z
DO  - 10.1016/j.celrep.2020.107952
EP  - 107952
PY  - 2020///
SN  - 2211-1247
SP  - 107952
TI  - Cycling Stem Cells Are Radioresistant and Regenerate the Intestine.
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2020.107952
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32726617
UR  - http://hdl.handle.net/10044/1/81341
VL  - 32
ER  -
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