Imperial College London
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ProfessorXiaodongZhang

Faculty of Medicine, Department of Infectious Disease

Professor of Macromolecular Structure and Function
 
 
 
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Contact

 

+44 (0)20 7594 3151xiaodong.zhang Website

 
 
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Assistant

 

Miss Kelly Butler +44 (0)20 7594 2763

 
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Location

 

104Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sawicka:2017:10.1016/bs.mie.2017.03.010,
author = {Sawicka, M and Aramayo, R and Ayala, R and Glyde, R and Zhang, X},
doi = {10.1016/bs.mie.2017.03.010},
journal = {Methods in Enzymology},
pages = {159--186},
title = {Single-Particle Electron Microscopy Analysis of DNA Repair Complexes},
url = {http://dx.doi.org/10.1016/bs.mie.2017.03.010},
volume = {592},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - DNA repair complexes play crucial roles in maintaining genome integrity, which is essential for the survival of an organism. The understanding of their modes of action is often obscure due to limited structural knowledge. Structural characterizations of these complexes are often challenging due to a poor protein production yield, a conformational flexibility, and a relatively high molecular mass. Single-particle electron microscopy (EM) has been successfully applied to study some of these complexes as it requires low amount of samples, is not limited by the high molecular mass of a protein or a complex, and can separate heterogeneous assemblies. Recently, near-atomic resolution structures have been obtained with EM owing to the advances in technology and image processing algorithms. In this chapter, we review the EM methodology of obtaining three-dimensional reconstructions of macromolecular complexes and provide a workflow that can be applied to DNA repair complex assemblies.
AU  - Sawicka,M
AU  - Aramayo,R
AU  - Ayala,R
AU  - Glyde,R
AU  - Zhang,X
DO  - 10.1016/bs.mie.2017.03.010
EP  - 186
PY  - 2017///
SN  - 0076-6879
SP  - 159
TI  - Single-Particle Electron Microscopy Analysis of DNA Repair Complexes
T2  - Methods in Enzymology
UR  - http://dx.doi.org/10.1016/bs.mie.2017.03.010
VL  - 592
ER  -
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