Publications
183 results found
Takahashi Y, Shevchuk AI, Novak P, et al., 2011, Multifunctional nanoprobes for nanoscale chemical imaging and localized chemical delivery at surfaces and interfaces., Angewandte Chemie (International ed. in English), Vol: 50, Pages: 9638-42, ISSN: 1521-3773
Klenerman D, Korchev YE, Davis SJ, 2011, Imaging and characterisation of the surface of live cells, CURRENT OPINION IN CHEMICAL BIOLOGY, Vol: 15, Pages: 696-703, ISSN: 1367-5931
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- Citations: 30
Latif L, Tribe IE, Babakinejad B, et al., 2011, Characterisation of pluripotent immortal postnatal mouse neural crest-like stem cells, PIGMENT CELL & MELANOMA RESEARCH, Vol: 24, Pages: 789-789, ISSN: 1755-1471
Shevchuk AI, Novak P, Takahashi Y, et al., 2011, Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe., Nanomedicine (London, England), Vol: 6, Pages: 565-75, ISSN: 1748-6963
Cells naturally operate on the nanoscale level, with molecules combining together to form complex molecular machines, which can work together to enable normal cell function or go wrong as in the case of many diseases. Visualizing these key processes on the nanoscale has been difficult and two main approaches have been used to date; nanometer resolution imaging of fixed cells using electron microscopy, or imaging live cells using optical or fluorescence microscopy, with a resolution of a few hundred nanometers. Scanning probe microscopy has the potential to allow live cells to be imaged at nanoscale resolution and a noncontact method based on the use of a nanopipette probe has been developed over the last 10 years that allows both topographic and functional imaging. The rapid progress in this area of research over the last 4 years is reviewed in this article, which shows that imaging of complex cellular structures and tissues is now possible and that these methods are now sufficiently mature to provide new insights into important diseases.
Ruenraroengsak P, Novak P, Berhanu D, et al., 2011, Respiratory epithelial cytotoxicity and membrane damage (holes) caused by amine-modified nanoparticles., Nanotoxicology, ISSN: 1743-5404
Abstract The respiratory epithelium is a significant target of inhaled, nano-sized particles, the biological reactivity of which will depend on its physicochemical properties. Surface-modified, 50 and 100 nm, polystyrene latex nanoparticles (NPs) were used as model particles to examine the effect of particle size and surface chemistry on transformed human alveolar epithelial type 1-like cells (TT1). Live images of TT1 exposed to amine-modified NPs taken by hopping probe ion conductance microscopy revealed severe damage and holes on cell membranes that were not observed with other types of NPs. This paralleled induction of cell detachment, cytotoxicity and apoptotic (caspase-3/7 and caspase-9) cell death, and increased release of CXCL8 (IL-8). In contrast, unmodified, carboxyl-modified 50 nm NPs and the 100 nm NPs did not cause membrane damage, and were less reactive. Thus, the susceptibility and membrane damage to respiratory epithelium following inhalation of NPs will depend on both surface chemistry (e.g., cationic) and nano-size.
Miragoli M, Moshkov A, Novak P, et al., 2011, Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells, Journal of the Royal Society Interface, Vol: 8, Pages: 913-925, ISSN: 1742-5662
Cardiovascular diseases are complex pathologies that include alterations of various cell functions at the levels of intact tissue, single cells and subcellular signalling compartments. Conventional techniques to study these processes are extremely divergent and rely on a combination of individual methods, which usually provide spatially and temporally limited information on single parameters of interest. This review describes scanning ion conductance microscopy (SICM) as a novel versatile technique capable of simultaneously reporting various structural and functional parameters at nanometre resolution in living cardiovascular cells at the level of the whole tissue, single cells and at the subcellular level, to investigate the mechanisms of cardiovascular disease. SICM is a multimodal imaging technology that allows concurrent and dynamic analysis of membrane morphology and various functional parameters (cell volume, membrane potentials, cellular contraction, single ion-channel currents and some parameters of intracellular signalling) in intact living cardiovascular cells and tissues with nanometre resolution at different levels of organization (tissue, cellular and subcellular levels). Using this technique, we showed that at the tissue level, cell orientation in the inner and outer aortic arch distinguishes atheroprone and atheroprotected regions. At the cellular level, heart failure leads to a pronounced loss of T-tubules in cardiac myocytes accompanied by a reduction in Z-groove ratio. We also demonstrated the capability of SICM to measure the entire cell volume as an index of cellular hypertrophy. This method can be further combined with fluorescence to simultaneously measure cardiomyocyte contraction and intracellular calcium transients or to map subcellular localization of membrane receptors coupled to cyclic adenosine monophosphate production. The SICM pipette can be used for patch-clamp recordings of membrane potential and single channel currents. In conclusio
Takahashi Y, Shevchuk AI, Novak P, et al., 2010, Simultaneous Noncontact Topography and Electrochemical Imaging by SECM/SICM Featuring Ion Current Feedback Regulation, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 132, Pages: 10118-10126, ISSN: 0002-7863
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- Citations: 231
Adler J, Shevchuk AI, Novak P, et al., 2010, Plasma membrane topography and interpretation of single-particle tracks., Nature methods, Vol: 7, Pages: 170-1, ISSN: 1548-7105
Nikolaev VO, Moshkov A, Lyon AR, et al., 2010, Beta2-adrenergic receptor redistribution in heart failure changes cAMP compartmentation., Science (New York, N.Y.), Vol: 327, Pages: 1653-7
The beta1- and beta2-adrenergic receptors (betaARs) on the surface of cardiomyocytes mediate distinct effects on cardiac function and the development of heart failure by regulating production of the second messenger cyclic adenosine monophosphate (cAMP). The spatial localization in cardiomyocytes of these betaARs, which are coupled to heterotrimeric guanine nucleotide-binding proteins (G proteins), and the functional implications of their localization have been unclear. We combined nanoscale live-cell scanning ion conductance and fluorescence resonance energy transfer microscopy techniques and found that, in cardiomyocytes from healthy adult rats and mice, spatially confined beta2AR-induced cAMP signals are localized exclusively to the deep transverse tubules, whereas functional beta1ARs are distributed across the entire cell surface. In cardiomyocytes derived from a rat model of chronic heart failure, beta2ARs were redistributed from the transverse tubules to the cell crest, which led to diffuse receptor-mediated cAMP signaling. Thus, the redistribution of beta(2)ARs in heart failure changes compartmentation of cAMP and might contribute to the failing myocardial phenotype.
Burkinshaw L, Clarke R, Novak P, et al., 2010, Surface Charge Mapping Based on Scanning Ion Conductance Microscopy, BIOPHYSICAL JOURNAL, Vol: 98, Pages: 394A-394A, ISSN: 0006-3495
Richards O, Johson N, Li C, et al., 2010, Probing the Interaction between a Nanopipette and a Soft Surface Using Scanning Ion Conductance Microscopy (SICM), BIOPHYSICAL JOURNAL, Vol: 98, Pages: 394A-394A, ISSN: 0006-3495
Sviderskaya EV, Easty DJ, Lawrence MA, et al., 2009, Functional neurons and melanocytes induced from immortal lines of postnatal neural crest-like stem cells, FASEB JOURNAL, Vol: 23, Pages: 3179-3192, ISSN: 0892-6638
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- Citations: 24
Banfic H, Visnjic D, Mise N, et al., 2009, Epidermal growth factor stimulates translocation of the class II phosphoinositide 3-kinase PI3K-C2β to the nucleus, BIOCHEMICAL JOURNAL, Vol: 422, Pages: 53-60, ISSN: 0264-6021
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- Citations: 15
Lyon AR, MacLeod KT, Zhang Y, et al., 2009, Loss of T-tubules and other changes to surface topography in ventricular myocytes from failing human and rat heart, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 106, Pages: 6854-6859, ISSN: 0027-8424
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- Citations: 279
Novak P, Li C, Shevchuk AI, et al., 2009, Nanoscale live-cell imaging using hopping probe ion conductance microscopy, Nature Methods, Vol: 6, Pages: 279-281, ISSN: 1548-7105
We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.
Novak P, Li C, Shevchuk A, et al., 2009, Next Generation SICM Allows Nanoscale Imaging Of Biological Processes In Real-time, Publisher: CELL PRESS, Pages: 374A-374A, ISSN: 0006-3495
Gorelik J, Ali NN, Kadir SHSA, et al., 2008, Non-invasive Imaging of Stem Cells by Scanning Ion Conductance Microscopy: Future Perspective, TISSUE ENGINEERING PART C-METHODS, Vol: 14, Pages: 311-318, ISSN: 1937-3384
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- Citations: 18
Kemp SJ, Thorley AJ, Gorelik J, et al., 2008, Immortalization of Human Alveolar Epithelial Cells to Investigate Nanoparticle Uptake, AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, Vol: 39, Pages: 591-597, ISSN: 1044-1549
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- Citations: 103
Anand U, Otto WR, Sanchez-Herrera D, et al., 2008, Cannabinoid receptor CB2 localisation and agonist-mediated inhibition of capsaicin responses in human sensory neurons, PAIN, Vol: 138, Pages: 667-680, ISSN: 0304-3959
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- Citations: 125
Sánchez D, Johnson N, Li C, et al., 2008, Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette., Biophysical journal, Vol: 95, Pages: 3017-27, ISSN: 1542-0086
Mechanosensitivity in living biological tissue is a study area of increasing importance, but investigative tools are often inadequate. We have developed a noncontact nanoscale method to apply quantified positive and negative force at defined positions to the soft responsive surface of living cells. The method uses applied hydrostatic pressure (0.1-150 kPa) through a pipette, while the pipette-sample separation is kept constant above the cell surface using ion conductance based distance feedback. This prevents any surface contact, or contamination of the pipette, allowing repeated measurements. We show that we can probe the local mechanical properties of living cells using increasing pressure, and hence measure the nanomechanical properties of the cell membrane and the underlying cytoskeleton in a variety of cells (erythrocytes, epithelium, cardiomyocytes and neurons). Because the cell surface can first be imaged without pressure, it is possible to relate the mechanical properties to the local cell topography. This method is well suited to probe the nanomechanical properties and mechanosensitivity of living cells.
Gorelik J, Yang L, Zhang Y, et al., 2008, WITHDRAWN: A novel Z-groove index characterizing myocardial surface structure and its modification in failing human heart., J Mol Cell Cardiol, ISSN: 1095-8584
The publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.yjmcc.2007.03.496. The duplicate article has therefore been withdrawn.
Piper JD, Li C, Lo C-J, et al., 2008, Characterization and application of controllable local chemical changes produced by reagent delivery from a nanopipet, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 130, Pages: 10386-10393, ISSN: 0002-7863
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- Citations: 31
Li C, Johnson N, Ostanin V, et al., 2008, High resolution imaging using scanning ion conductance microscopy with improved distance feedback control, PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL, Vol: 18, Pages: 671-677, ISSN: 1002-0071
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- Citations: 24
Shevchuk AI, Hobson P, Lab MJ, et al., 2008, Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy, BIOPHYSICAL JOURNAL, Vol: 94, Pages: 4089-4094, ISSN: 0006-3495
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- Citations: 36
Sviderskaya EV, Negulyaev YA, Sanchez D, et al., 2008, Functional characterisation of neurons produced by postnatal neural crest-like stem cells, PIGMENT CELL & MELANOMA RESEARCH, Vol: 21, Pages: 311-311, ISSN: 1755-1471
Shevchuk AI, Hobson P, Lab MJ, et al., 2008, Endocytic pathways: combined scanning ion conductance and surface confocal microscopy study, PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, Vol: 456, Pages: 227-235, ISSN: 0031-6768
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- Citations: 35
Dutta AK, Korchev YE, Shevchuk AI, et al., 2008, Spatial distribution of maxi-anion channel on cardiomyocytes detected by smart-patch technique, BIOPHYSICAL JOURNAL, Vol: 94, Pages: 1646-1655, ISSN: 0006-3495
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- Citations: 43
Zhang Y, Ma L, Xu B, et al., 2008, PI3K-Mediated Epithelial Sodium Channel Activity by Regulating the Apical Membrane Morphology of Renal Epithelial Cells, 7th Asia-Pacific Conference on Medical and Biological Engineering, Publisher: SPRINGER, Pages: 603-+, ISSN: 1680-0737
Bruckbauer A, James P, Zhou D, et al., 2007, Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking, BIOPHYSICAL JOURNAL, Vol: 93, Pages: 3120-3131, ISSN: 0006-3495
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- Citations: 81
Zhang Y, Sanchez D, Gorelik J, et al., 2007, Basolateral P2X<sub>4</sub>-like receptors regulate the extracellular ATP-stimulated epithelial Na<SUP>+</SUP> channel activity in renal epithelia, AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, Vol: 292, Pages: F1734-F1740, ISSN: 1931-857X
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- Citations: 35
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