Imperial College London
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ProfessorYuriKorchev

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Biophysics
 
 
 
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Contact

 

+44 (0)20 3313 3080y.korchev

 
 
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Location

 

BN5, Nanomedicine LabCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bednarska:2020:10.1111/jmi.12972,
author = {Bednarska, J and Novak, P and Korchev, Y and Rorsman, P and Tarasov, AI and Shevchuk, A},
doi = {10.1111/jmi.12972},
journal = {Journal of Microscopy},
pages = {21--29},
title = {Release of insulin granules by simultaneous, high-speed correlative SICM-FCM},
url = {http://dx.doi.org/10.1111/jmi.12972},
volume = {282},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Exocytosis of peptides and steroids stored in a dense core vesicular (DCV) form is the final step of every secretory pathway, indispensable for the function of nervous, endocrine and immune systems. The lack of live imaging techniques capable of direct, labelfree visualisation of DCV release makes many aspects of the exocytotic process inaccessible to investigation. We describe the application of correlative scanning ion conductance and fluorescence confocal microscopy (SICMFCM) to study the exocytosis of individual granules of insulin from the top, nonadherent, surface of pancreatic βcells. Using SICMFCM, we were first to directly follow the topographical changes associated with physiologically induced release of insulin DCVs. This allowed us to report the kinetics of the full fusion of the insulin vesicle as well as the subsequent solubilisation of the released insulin crystal.
AU  - Bednarska,J
AU  - Novak,P
AU  - Korchev,Y
AU  - Rorsman,P
AU  - Tarasov,AI
AU  - Shevchuk,A
DO  - 10.1111/jmi.12972
EP  - 29
PY  - 2020///
SN  - 0022-2720
SP  - 21
TI  - Release of insulin granules by simultaneous, high-speed correlative SICM-FCM
T2  - Journal of Microscopy
UR  - http://dx.doi.org/10.1111/jmi.12972
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000583504000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/10.1111/jmi.12972
UR  - http://hdl.handle.net/10044/1/88278
VL  - 282
ER  -
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