Imperial College London
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ProfessorYuriKorchev

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Biophysics
 
 
 
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Contact

 

+44 (0)20 3313 3080y.korchev

 
 
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Location

 

BN5, Nanomedicine LabCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kolmogorov:2021:10.1039/d0nr08349f,
author = {Kolmogorov, VS and Erofeev, AS and Woodcock, E and Efremov, YM and Iakovlev, AP and Savin, NA and Alova, AV and Lavrushkina, SV and Kireev, II and Prelovskaya, AO and Sviderskaya, EV and Scaini, D and Klyachko, NL and Timashev, PS and Takahashi, Y and Salikhov, SV and Parkhomenko, YN and Majouga, AG and Edwards, CRW and Novak, P and Korchev, YE and Gorelkin, PV},
doi = {10.1039/d0nr08349f},
journal = {Nanoscale},
pages = {6558--6568},
title = {Mapping mechanical properties of living cells at nanoscale using intrinsic nanopipette-sample force interactions dagger},
url = {http://dx.doi.org/10.1039/d0nr08349f},
volume = {13},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Mechanical properties of living cells determined by cytoskeletal elements play a crucial role in a wide range of biological functions. However, low-stress mapping of mechanical properties with nanoscale resolution but with a minimal effect on the fragile structure of cells remains difficult. Scanning Ion-Conductance Microscopy (SICM) for quantitative nanomechanical mapping (QNM) is based on intrinsic force interactions between nanopipettes and samples and has been previously suggested as a promising alternative to conventional techniques. In this work, we have provided an alternative estimation of intrinsic force and stress and demonstrated the possibility to perform qualitative and quantitative analysis of cell nanomechanical properties of a variety of living cells. Force estimation on decane droplets with well-known elastic properties, similar to living cells, revealed that the forces applied using a nanopipette are much smaller than in the case using atomic force microscopy. We have shown that we can perform nanoscale topography and QNM using a scanning procedure with no detectable effect on live cells, allowing long-term QNM as well as detection of nanomechanical properties under drug-induced alterations of actin filaments and microtubulin.
AU  - Kolmogorov,VS
AU  - Erofeev,AS
AU  - Woodcock,E
AU  - Efremov,YM
AU  - Iakovlev,AP
AU  - Savin,NA
AU  - Alova,AV
AU  - Lavrushkina,SV
AU  - Kireev,II
AU  - Prelovskaya,AO
AU  - Sviderskaya,EV
AU  - Scaini,D
AU  - Klyachko,NL
AU  - Timashev,PS
AU  - Takahashi,Y
AU  - Salikhov,SV
AU  - Parkhomenko,YN
AU  - Majouga,AG
AU  - Edwards,CRW
AU  - Novak,P
AU  - Korchev,YE
AU  - Gorelkin,PV
DO  - 10.1039/d0nr08349f
EP  - 6568
PY  - 2021///
SN  - 2040-3364
SP  - 6558
TI  - Mapping mechanical properties of living cells at nanoscale using intrinsic nanopipette-sample force interactions dagger
T2  - Nanoscale
UR  - http://dx.doi.org/10.1039/d0nr08349f
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000635278300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/NR/D0NR08349F#!divAbstract
VL  - 13
ER  -
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