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@article{Zhang:2016:10.1038/srep37988,
author = {Zhang, Y and Fear, D and Willis-Owen, S and Cookson, W and Moffatt, M},
doi = {10.1038/srep37988},
journal = {Scientific Reports},
title = {Global gene regulation during activation of immunoglobulin class switching in human B cells},
url = {http://dx.doi.org/10.1038/srep37988},
volume = {6},
year = {2016}
}

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TY  - JOUR
AB  - Immunoglobulin class switch recombination (CSR) to IgE is a tightly regulated process central to atopic disease. To profile the B-cell transcriptional responses underlying the activation of the germinal centre activities leading to the generation of IgE, naïve human B-cells were stimulated with IL-4 and anti-CD40. Gene expression and alternative splicing were profiled over 12 days using the Affymetrix Human Exon 1.0 ST Array. A total of 1,399 genes, forming 13 temporal profiles were differentially expressed. CCL22 and CCL17 were dramatically induced but followed a temporal trajectory distinct from classical mediators of isotype switching. AICDA, NFIL3, IRF4, XBP1 and BATF3 shared a profile with several genes involved in innate immunity, but with no recognised role in CSR. A transcription factor BHLHE40 was identified at the core of this profile. B-cell activation was also accompanied by variation in exon retention affecting >200 genes including CCL17. The data indicate a circadian component and central roles for the Th2 chemokines CCL22 and CCL17 in the activation of CSR.
AU  - Zhang,Y
AU  - Fear,D
AU  - Willis-Owen,S
AU  - Cookson,W
AU  - Moffatt,M
DO  - 10.1038/srep37988
PY  - 2016///
SN  - 2045-2322
TI  - Global gene regulation during activation of immunoglobulin class switching in human B cells
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep37988
UR  - http://hdl.handle.net/10044/1/42297
VL  - 6
ER  -

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