Imperial College London
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DrYanLiu

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Glycosciences
 
 
 
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Contact

 

yan.liu2 Website

 
 
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Location

 

E518Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Vendele:2019:10.1101/677104,
author = {Vendele, I and Willment, JA and Silva, LM and Palma, AS and Chai, W and Liu, Y and Feizi, T and Stappers, MHT and Brown, GD and Gow, NAR},
doi = {10.1101/677104},
title = {Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls},
url = {http://dx.doi.org/10.1101/677104},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>During the course of fungal infection, pathogen recognition by the innate immune system is critical to initiate efficient protective immune responses. The primary event that triggers immune responses is the binding of Pattern Recognition Receptors (PRRs), which are expressed at the surface of host immune cells, to Pathogen-Associated Molecular Patterns (PAMPs) located predominantly in the fungal cell wall. Most fungi have mannosylated PAMPs in their cell walls and these are recognized by a range of C-type lectin receptors (CTLs). However, the precise spatial distribution of the ligands that induce immune responses within the cell walls of fungi are not well defined. We used recombinant IgG Fc-CTLs fusions of three murine mannan detecting CTLs, including dectin-2, the mannose receptor (MR) carbohydrate recognition domains (CRDs) 4-7 (CRD4-7), and human DC-SIGN (hDC-SIGN) and the β-1,3 glucan-binding lectin dectin-1 to map PRR ligands in the fungal cell wall. We show that epitopes of mannan-specific CTL receptors can be clustered or diffuse, superficial or buried in the inner cell wall. We demonstrate that PRR ligands do not correlate well with phylogenetic relationships between fungi, and that Fc-lectin binding discriminated between mannosides expressed on different cell morphologies of the same fungus. We also demonstrate CTL epitope differentiation during different phases of the growth cycle of<jats:italic>Candida albicans</jats:italic>and that MR and DC-SIGN labelled outer chain<jats:italic>N</jats:italic>-mannans whilst dectin-2 labelled core<jats:italic>N</jats:italic>-mannans displayed deeper in the cell wall. These immune receptor maps of fungal walls therefore reveal remarkable spatial, temporal and chemical diversity, indicating that the triggering of immune recognition events originates from multiple physical origins at the fungal cell surface.</jats:p>
AU  - Vendele,I
AU  - Willment,JA
AU  - Silva,LM
AU  - Palma,AS
AU  - Chai,W
AU  - Liu,Y
AU  - Feizi,T
AU  - Stappers,MHT
AU  - Brown,GD
AU  - Gow,NAR
DO  - 10.1101/677104
PY  - 2019///
TI  - Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls
UR  - http://dx.doi.org/10.1101/677104
ER  -
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