Imperial College London
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DrYanLiu

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Glycosciences
 
 
 
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Contact

 

yan.liu2 Website

 
 
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Location

 

E518Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sorin:2023:10.1016/j.celrep.2023.112114,
author = {Sorin, MN and Di, Maio A and Silva, LM and Ebert, D and Delannoy, CP and Nguyen, N-K and Guerardel, Y and Chai, W and Halary, F and Renaudin-Autain, K and Liu, Y and Bressollette-Bodin, C and Stehle, T and McIlroy, D},
doi = {10.1016/j.celrep.2023.112114},
journal = {Cell Reports},
pages = {1--18},
title = {Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus},
url = {http://dx.doi.org/10.1016/j.celrep.2023.112114},
volume = {42},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BK polyomavirus (BKPyV) is an opportunistic pathogen that uses the b-series gangliosides GD1b and GT1b as entry receptors. Here, we characterize the impact of naturally occurring VP1 mutations on ganglioside binding, VP1 protein structure, and virus tropism. Infectious entry of single mutants E73Q and E73A and the triple mutant A72V-E73Q-E82Q (VQQ) remains sialic acid dependent, and all three variants acquire binding to a-series gangliosides, including GD1a. However, the E73A and VQQ variants lose the ability to infect ganglioside-complemented cells, and this correlates with a clear shift of the BC2 loop in the crystal structures of E73A and VQQ. On the other hand, the K69N mutation in the K69N-E82Q variant leads to a steric clash that precludes sialic acid binding. Nevertheless, this mutant retains significant infectivity in 293TT cells, which is not dependent on heparan sulfate proteoglycans, implying that an unknown sialic acid-independent entry receptor for BKPyV exists.
AU  - Sorin,MN
AU  - Di,Maio A
AU  - Silva,LM
AU  - Ebert,D
AU  - Delannoy,CP
AU  - Nguyen,N-K
AU  - Guerardel,Y
AU  - Chai,W
AU  - Halary,F
AU  - Renaudin-Autain,K
AU  - Liu,Y
AU  - Bressollette-Bodin,C
AU  - Stehle,T
AU  - McIlroy,D
DO  - 10.1016/j.celrep.2023.112114
EP  - 18
PY  - 2023///
SN  - 2211-1247
SP  - 1
TI  - Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2023.112114
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36790933
UR  - https://www.cell.com/cell-reports/fulltext/S2211-1247(23)00125-0
UR  - http://hdl.handle.net/10044/1/103054
VL  - 42
ER  -
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