Imperial College London
Alternate

ProfessorZoltanTakats

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Analytical Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 2760z.takats

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Antonowicz:2018:10.1016/j.talanta.2018.08.029,
author = {Antonowicz, S and Hanna, GB and Takats, Z and Bodai, Z},
doi = {10.1016/j.talanta.2018.08.029},
journal = {Talanta},
pages = {436--442},
title = {Pragmatic and rapid analysis of carbonyl, oxidation and chlorination nucleoside-adducts in murine tissue by UPLC-ESI-MS/MS},
url = {http://dx.doi.org/10.1016/j.talanta.2018.08.029},
volume = {190},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Nucleoside-adduct analysis by liquid chromatography mass spectrometry is a powerful tool in genotoxicity studies. Efforts to date have quantified an impressive array of DNA damage products, although methodological diversity suggests quantification is still a challenging task. For example, inadequate co-examination of normal nucleosides, cumbersome sample preparation and large DNA requirements were identified to be recurring issues. A six-minute ultra-performance liquid chromatography method is presented which adequately separates seven candidate nucleoside-adducts from the four unmodified nucleosides. The method was sensitive to 1 adduct per 108 normal bases with 20µg DNA input for most targets. The method was shown to be accurate (81–119% across quintuplets of six tissue types) and precise (relative standard deviation 4–13%). The fast method time facilitated a second quantitation for normal nucleosides at an appropriate dilution, allowing DNA damage concentrations to be contextualised accurately sample-to-sample. From DNA samples, the analytical processing time was <8h, and 96 samples can easily be prepared in a day. The method was used to quantify carbonyl, chloro- and oxo- adducts in murine tissue samples.
AU  - Antonowicz,S
AU  - Hanna,GB
AU  - Takats,Z
AU  - Bodai,Z
DO  - 10.1016/j.talanta.2018.08.029
EP  - 442
PY  - 2018///
SN  - 0039-9140
SP  - 436
TI  - Pragmatic and rapid analysis of carbonyl, oxidation and chlorination nucleoside-adducts in murine tissue by UPLC-ESI-MS/MS
T2  - Talanta
UR  - http://dx.doi.org/10.1016/j.talanta.2018.08.029
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000445306500056&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S0039914018308403?via%3Dihub
UR  - http://hdl.handle.net/10044/1/79017
VL  - 190
ER  -
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