Imperial College London
Alternate

ProfessorZoltanTakats

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Analytical Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 2760z.takats

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Strittmatter:2021:10.1021/jasms.1c00240,
author = {Strittmatter, N and Kanvatirth, P and Inglese, P and Race, AM and Nilsson, A and Dannhorn, A and Kudo, H and Goldin, RD and Ling, S and Wong, E and Seeliger, F and Serra, MP and Hoffmann, S and Maglennon, G and Hamm, G and Atkinson, J and Jones, S and Bunch, J and Andrén, PE and Takats, Z and Goodwin, RJA and Mastroeni, P},
doi = {10.1021/jasms.1c00240},
journal = {Journal of the American Society for Mass Spectrometry},
pages = {2791--2802},
title = {Holistic characterization of a salmonella typhimurium infection model using integrated molecular imaging.},
url = {http://dx.doi.org/10.1021/jasms.1c00240},
volume = {32},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A more complete and holistic view on host-microbe interactions is needed to understand the physiological and cellular barriers that affect the efficacy of drug treatments and allow the discovery and development of new therapeutics. Here, we developed a multimodal imaging approach combining histopathology with mass spectrometry imaging (MSI) and same section imaging mass cytometry (IMC) to study the effects of Salmonella Typhimurium infection in the liver of a mouse model using the S. Typhimurium strains SL3261 and SL1344. This approach enables correlation of tissue morphology and specific cell phenotypes with molecular images of tissue metabolism. IMC revealed a marked increase in immune cell markers and localization in immune aggregates in infected tissues. A correlative computational method (network analysis) was deployed to find metabolic features associated with infection and revealed metabolic clusters of acetyl carnitines, as well as phosphatidylcholine and phosphatidylethanolamine plasmalogen species, which could be associated with pro-inflammatory immune cell types. By developing an IMC marker for the detection of Salmonella LPS, we were further able to identify and characterize those cell types which contained S. Typhimurium.
AU  - Strittmatter,N
AU  - Kanvatirth,P
AU  - Inglese,P
AU  - Race,AM
AU  - Nilsson,A
AU  - Dannhorn,A
AU  - Kudo,H
AU  - Goldin,RD
AU  - Ling,S
AU  - Wong,E
AU  - Seeliger,F
AU  - Serra,MP
AU  - Hoffmann,S
AU  - Maglennon,G
AU  - Hamm,G
AU  - Atkinson,J
AU  - Jones,S
AU  - Bunch,J
AU  - Andrén,PE
AU  - Takats,Z
AU  - Goodwin,RJA
AU  - Mastroeni,P
DO  - 10.1021/jasms.1c00240
EP  - 2802
PY  - 2021///
SN  - 1044-0305
SP  - 2791
TI  - Holistic characterization of a salmonella typhimurium infection model using integrated molecular imaging.
T2  - Journal of the American Society for Mass Spectrometry
UR  - http://dx.doi.org/10.1021/jasms.1c00240
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34767352
UR  - https://pubs.acs.org/doi/10.1021/jasms.1c00240
UR  - http://hdl.handle.net/10044/1/93662
VL  - 32
ER  -
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