Imperial College London
Alternate

ProfessorZoltanTakats

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Analytical Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 2760z.takats

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wu:2022:10.1021/acs.analchem.2c00345,
author = {Wu, V and Tillner, J and Jones, E and McKenzie, JS and Gurung, D and Mroz, A and Poynter, L and Simon, D and Grau, C and Altafaj, X and Dumas, M-E and Gilmore, I and Bunch, J and Takats, Z},
doi = {10.1021/acs.analchem.2c00345},
journal = {Analytical Chemistry},
pages = {10035--10044},
title = {High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization},
url = {http://dx.doi.org/10.1021/acs.analchem.2c00345},
volume = {94},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In this study, we examine the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue. We also compare the performance of desorption electrospray ionization (DESI) with and without the flow focusing setup. The main potential advantages of applying the flow focusing mechanism in DESI is its rotationally symmetric electrospray jet, higher intensity, more controllable parameters, and better portability due to the robustness of the sprayer. The parameters for DEFFI have therefore been thoroughly optimized, primarily for spatial resolution but also for intensity. Once the parameters have been optimized, DEFFI produces similar images to the existing DESI. MS images for mouse brain samples, acquired at a nominal pixel size of 50 μm, are comparable for both DESI setups, albeit the new sprayer design yields better sensitivity. Furthermore, the two methods are compared with regard to spectral intensity as well as the area of the desorbed crater on rhodamine-coated slides. Overall, the implementation of a flow focusing mechanism in DESI is shown to be highly suitable for imaging biological tissue and has potential to overcome some of the shortcomings experienced with the current geometrical design of DESI.
AU  - Wu,V
AU  - Tillner,J
AU  - Jones,E
AU  - McKenzie,JS
AU  - Gurung,D
AU  - Mroz,A
AU  - Poynter,L
AU  - Simon,D
AU  - Grau,C
AU  - Altafaj,X
AU  - Dumas,M-E
AU  - Gilmore,I
AU  - Bunch,J
AU  - Takats,Z
DO  - 10.1021/acs.analchem.2c00345
EP  - 10044
PY  - 2022///
SN  - 0003-2700
SP  - 10035
TI  - High resolution ambient MS imaging of biological samples by desorption electro-flow focussing ionization
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.2c00345
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000826859200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.2c00345
UR  - http://hdl.handle.net/10044/1/99120
VL  - 94
ER  -
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