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@article{Ramuz:2022:10.1007/978-1-0716-2245-2_3,
author = {Ramuz, M and Diakonov, I and Dunsby, C and Gorelik, J},
doi = {10.1007/978-1-0716-2245-2_3},
journal = {Methods Mol Biol},
pages = {33--59},
title = {MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRET.},
url = {http://dx.doi.org/10.1007/978-1-0716-2245-2_3},
volume = {2483},
year = {2022}
}
TY - JOUR
AB - The newly generated software plugin MultiFRET allows for real-time measurements of multiplexed fluorescent biosensors in a near high-throughput fashion. Here we describe a detailed protocol for setup and use of this software for any purpose requiring instant feedback during fluorescence measurement experiments. We further describe its non-primary features including beam splitter misalignment correction, custom calculations through input of simple equations typed in a .txt format, customizable Excel output, and offline bulk analysis of image stacks. Finally, we supply a usage example of a cAMP measurement in cultured rat neonatal cardiomyocytes.
AU - Ramuz,M
AU - Diakonov,I
AU - Dunsby,C
AU - Gorelik,J
DO - 10.1007/978-1-0716-2245-2_3
EP - 59
PY - 2022///
SP - 33
TI - MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRET.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1007/978-1-0716-2245-2_3
UR - https://www.ncbi.nlm.nih.gov/pubmed/35286668
VL - 2483
ER -