Publications

See a list of publications below or visit the Photonics academic staff page and click on a particular  member of staff to access their personal web page, which includes a list of their own publications.

Citation

BibTex format

@article{Myatt:2013:10.1038/onc.2013.546,
author = {Myatt, SS and Kongsema, M and Man, CW-Y and Kelly, DJ and Gomes, AR and Khongkow, P and Karunarathna, U and Zona, S and Langer, JK and Dunsby, CW and Coombes, RC and French, PM and Brosens, JJ and Lam, EW-F},
doi = {10.1038/onc.2013.546},
journal = {Oncogene},
pages = {4316--4329},
title = {SUMOylation inhibits FOXM1 activity and delays mitotic transition},
url = {http://dx.doi.org/10.1038/onc.2013.546},
volume = {33},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The forkhead box transcription factor FOXM1 is an essential effector of G2/M-phase transition, mitosis and the DNA damage response. As such, it is frequently deregulated during tumorigenesis. Here we report that FOXM1 is dynamically modified by SUMO1 but not by SUMO2/3 at multiple sites. We show that FOXM1 SUMOylation is enhanced in MCF-7 breast cancer cells in response to treatment with epirubicin and mitotic inhibitors. Mutation of five consensus conjugation motifs yielded a SUMOylation-deficient mutant FOXM1. Conversely, fusion of the E2 ligase Ubc9 to FOXM1 generated an auto-SUMOylating mutant (FOXM1-Ubc9). Analysis of wild-type FOXM1 and mutants revealed that SUMOylation inhibits FOXM1 activity, promotes translocation to the cytoplasm and enhances APC/Cdh1-mediated ubiquitination and degradation. Further, expression of the SUMOylation-deficient mutant enhanced cell proliferation compared with wild-type FOXM1, whereas the FOXM1-Ubc9 fusion protein resulted in persistent cyclin B1 expression and slowed the time from mitotic entry to exit. In summary, our findings suggest that SUMOylation attenuates FOXM1 activity and causes mitotic delay in cytotoxic drug response.
AU  - Myatt,SS
AU  - Kongsema,M
AU  - Man,CW-Y
AU  - Kelly,DJ
AU  - Gomes,AR
AU  - Khongkow,P
AU  - Karunarathna,U
AU  - Zona,S
AU  - Langer,JK
AU  - Dunsby,CW
AU  - Coombes,RC
AU  - French,PM
AU  - Brosens,JJ
AU  - Lam,EW-F
DO  - 10.1038/onc.2013.546
EP  - 4329
PY  - 2013///
SN  - 1476-5594
SP  - 4316
TI  - SUMOylation inhibits FOXM1 activity and delays mitotic transition
T2  - Oncogene
UR  - http://dx.doi.org/10.1038/onc.2013.546
UR  - http://hdl.handle.net/10044/1/39236
VL  - 33
ER  -
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