@article{Lopez-Garrido:2018:10.1016/j.cell.2018.01.027,
author = {Lopez-Garrido, J and Ojkic, N and Khanna, K and Wagner, FR and Villa, E and Endres, RG and Pogliano, K},
doi = {10.1016/j.cell.2018.01.027},
journal = {Cell},
pages = {758--770.e14},
title = {Chromosome translocation inflates bacillus forespores and impacts cellular morphology},
url = {http://dx.doi.org/10.1016/j.cell.2018.01.027},
volume = {172},
year = {2018}
}
TY - JOUR
AB - The means by which the physicochemical properties of different cellular components together determine bacterial cell shape remain poorly understood. Here, we investigate a programmed cell-shape change during Bacillus subtilis sporulation, when a rod-shaped vegetative cell is transformed to an ovoid spore. Asymmetric cell division generates a bigger mother cell and a smaller, hemispherical forespore. The septum traps the forespore chromosome, which is translocated to the forespore by SpoIIIE. Simultaneously, forespore size increases as it is reshaped into an ovoid. Using genetics, timelapse microscopy, cryo-electron tomography, and mathematical modeling, we demonstrate that forespore growth relies on membrane synthesis and SpoIIIE-mediated chromosome translocation, but not on peptidoglycan or protein synthesis. Our data suggest that the hydrated nucleoid swells and inflates the forespore, displacing ribosomes to the cell periphery, stretching septal peptidoglycan, and reshaping the forespore. Our results illustrate how simple biophysical interactions between core cellular components contribute to cellular morphology.
AU - Lopez-Garrido,J
AU - Ojkic,N
AU - Khanna,K
AU - Wagner,FR
AU - Villa,E
AU - Endres,RG
AU - Pogliano,K
DO - 10.1016/j.cell.2018.01.027
EP - 770
PY - 2018///
SN - 0092-8674
SP - 758
TI - Chromosome translocation inflates bacillus forespores and impacts cellular morphology
T2 - Cell
UR - http://dx.doi.org/10.1016/j.cell.2018.01.027
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000424648800016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/67348
VL - 172
ER -