The application of metabonomics in a clinical and surgical setting was first introduced by Professor Jeremy Nicholson et al. (Nature 491, 384–392). The CPC conducts research with the aim of achieving patient stratification through metabolic phenotyping. The resulting metabolic profiles contain diagnostic and mechanistic information which can be translated to improved patient care. The studies analysed at the Centre cover a spectrum of diseases including cancer, parasitic infections, viral infections, allergy and neurological conditions.
In parallel to patient phenotyping, the CPC, within the academic section of Computational and Systems Medicine (CSM), is continuously developing the clinical application of metabonomics through the use of global metabolic profiling of novel sample matrices or the introduction of novel validated targeted methods.
A list of selected publications showing how metabonomics is applied in stratified medicine is presented below.
Aurelien A, Dona CA, Wijeyesekera A, et al. 2015, 1H NMR Spectroscopy of faecal Extracts enables detection of Colorectal Neoplasia, Journal of Proteome Research, Vol:14, Pages:3871-3881. PMID: 26211820
Correia GDS, Ng KW, Wijeyesekera A, et al. 2015, Metabolic profiling of children undergoing surgery for congenital heart disease, Critical Care Medicine, Vol:43, Pages:1467-1476. PMID: 25844698
Villasenor A, Kinross JM, Li JV, et al. 2014, 1H NMR global metabolic phenotyping of acute pancreatitis in the emergency unit, Journal of Proteome Research, Vol:13, Pages:5362-5375. PMID: 25160714
Mirnezami R, Spagou K, Vorkas PA, et al. 2014, Chemical mapping of the colorectal cancer microenvironment via MALDI imaging mass spectrometry (MALDI-MSI) reveals novel cancer-associated field effects, Molecular Oncology, Vol:8, Pages:39-49. PMID: 24112879
Mirnezami R, Jimenez B, Li JV, et al., 2014, Rapid diagnosis and staging of colorectal cancer via high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy of intact tissue biopsies, Annals of Surgery, Vol:259, Pages:1138-1149. PMID: 23860197
Jimenez B, Mirnezami R, Kinross J, et al., 2013, 1H HR-MAS NMR Spectroscopy of tumor-induced local metabolic "field-effects" enables colorectal cancer staging and prognostication, Journal of Proteome Research, Vol:12, Pages:959-968. PMID: 23240862
Lewis MR, Pearce JTM, Spagou K, et al. 2016 Development and application of ultra-performance liquid chromatography-TOF MS for precision large scale urinary metabolic phenotyping, Analytical Chemistry, Vol:88, Pages: 9004–9013. PMID: 27479709
Anwar M, Vorkas P, Li J, et al. 2015 Optimization of metabolite extraction of human vein tissue for ultra performance liquid chromatography-mass spectrometry and nuclear magnetic resonance-based untargeted metabolic profiling, Analyst, Vol:140, Pages: 7586-7597. PMID: 26468486
Sarafian MH, Gaudin M, Lewis MR, et al. 2014, Objective set of criteria for optimization of sample preparation procedures for ultra-high throughput untargeted blood plasma lipid profiling by Ultra Performance Liquid Chromatography–Mass Spectrometry, Analytical Chemistry, Vol:86, Pages: 5766–5774. PMID: 24820162
Dona AC., Jimenez B, Schaefer H, et al. 2014, Precision high throughput proton NMR spectroscopy of human urine, serum and plasma for large-scale metabolic phenotyping. Analytical Chemistry, Vol:86, Pages: 9887-9894. PMID: 25180432
Want EJ, Masson P, Michopoulos F, et al., 2013, Global metabolic profiling of animal and human tissues via UPLC-MS, Nature Protocols, Vol:8, Pages:17-32. PMID: 23222455
Balog J, Sasi-Szabo L, Kinross J, et al., 2013, Intraoperative tissue identification using rapid evaporative ionisation mass spectrometry, Science Translational Medicine, Vol:5. PMID: 23863833
Wong A, Jimenez B, Li X, et al., 2012, Evaluation of High-Resolution Magic-Angle Coil Spinning NMR Spectroscopy for metabolic profiling of nanoliter tissue biopsies, Analytical Chemistry, Vol:84, Pages:3843-3848. PMID: 22449140
Beckonert O, Coen M, Keun HC, et al., 2010, High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues, Nature Protocols, Vol:5, Pages:1019-1032. PMID: 20539278
Want EJ, Wilson ID, Gika H, et al., 2010, Global metabolic profiling procedures for urine using UPLC-MS, Nature Protocols, Vol:5, Pages:1005-1018. PMID: 20448546
Beckonert O, Keun HC, Ebbels TMD, et al., 2007, Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts, Nature Protocols, Vol:2, Pages:2692-2703. PMID: 18007604