The application of metabonomics in a clinical and surgical setting was first introduced by Professor Jeremy Nicholson et al. (Nature 491, 384–392). The CPC conducts research with the aim of achieving patient stratification through metabolic phenotyping. The resulting metabolic profiles contain diagnostic and mechanistic information which can be translated to improved patient care. The studies analysed at the Centre cover a spectrum of diseases including cancer, parasitic infections, viral infections, allergy and neurological conditions.

In parallel to patient phenotyping, the CPC, within the academic section of Computational and Systems Medicine (CSM), is continuously developing the clinical application of metabonomics through the use of global metabolic profiling of novel sample matrices or the introduction of novel validated targeted methods. 

A list of selected publications showing how metabonomics is applied in stratified medicine is presented below.

Published research

Patient stratification

Aurelien A, Dona CA, Wijeyesekera A, et al. 2015, 1H NMR Spectroscopy of faecal Extracts enables detection of Colorectal NeoplasiaJournal of Proteome Research, Vol:14, Pages:3871-3881. PMID: 26211820

Correia GDS, Ng KW, Wijeyesekera A, et al. 2015, Metabolic profiling of children undergoing surgery for congenital heart disease, Critical Care Medicine, Vol:43, Pages:1467-1476. PMID: 25844698

Villasenor A, Kinross JM, Li JV, et al. 2014, 1H NMR global metabolic phenotyping of acute pancreatitis in the emergency unitJournal of Proteome Research, Vol:13, Pages:5362-5375. PMID: 25160714

Mirnezami R, Spagou K, Vorkas PA, et al. 2014, Chemical mapping of the colorectal cancer microenvironment via MALDI imaging mass spectrometry (MALDI-MSI) reveals novel cancer-associated field effectsMolecular Oncology, Vol:8, Pages:39-49. PMID: 24112879 

Mirnezami R, Jimenez B, Li JV, et al., 2014, Rapid diagnosis and staging of colorectal cancer via high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy of intact tissue biopsiesAnnals of Surgery, Vol:259, Pages:1138-1149. PMID: 23860197

Jimenez B, Mirnezami R, Kinross J, et al., 2013, 1H HR-MAS NMR Spectroscopy of tumor-induced local metabolic "field-effects" enables colorectal cancer staging and prognosticationJournal of Proteome Research, Vol:12, Pages:959-968. PMID: 23240862

Methods

Lewis MR, Pearce JTM, Spagou K, et al. 2016 Development and application of ultra-performance liquid chromatography-TOF MS for precision large scale urinary metabolic phenotyping, Analytical Chemistry, Vol:88, Pages: 9004–9013. PMID: 27479709

Anwar M, Vorkas P, Li J, et al. 2015 Optimization of metabolite extraction of human vein tissue for ultra performance liquid chromatography-mass spectrometry and nuclear magnetic resonance-based untargeted metabolic profilingAnalyst, Vol:140, Pages: 7586-7597. PMID: 26468486

Sarafian MH, Gaudin M, Lewis MR, et al. 2014, Objective set of criteria for optimization of sample preparation procedures for ultra-high throughput untargeted blood plasma lipid profiling by Ultra Performance Liquid Chromatography–Mass Spectrometry, Analytical Chemistry, Vol:86, Pages: 5766–5774. PMID: 24820162

Dona AC., Jimenez B, Schaefer H, et al. 2014, Precision high throughput proton NMR spectroscopy of human urine, serum and plasma for large-scale metabolic phenotyping. Analytical Chemistry, Vol:86, Pages: 9887-9894. PMID: 25180432

Want EJ, Masson P, Michopoulos F, et al., 2013, Global metabolic profiling of animal and human tissues via UPLC-MSNature Protocols, Vol:8, Pages:17-32. PMID: 23222455

Balog J, Sasi-Szabo L, Kinross J, et al., 2013, Intraoperative tissue identification using rapid evaporative ionisation mass spectrometryScience Translational Medicine, Vol:5. PMID: 23863833

Wong A, Jimenez B, Li X, et al., 2012, Evaluation of High-Resolution Magic-Angle Coil Spinning NMR Spectroscopy for metabolic profiling of nanoliter tissue biopsiesAnalytical Chemistry, Vol:84, Pages:3843-3848. PMID: 22449140

Beckonert O, Coen M, Keun HC, et al., 2010, High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissuesNature Protocols, Vol:5, Pages:1019-1032. PMID: 20539278

Want EJ, Wilson ID, Gika H, et al., 2010, Global metabolic profiling procedures for urine using UPLC-MSNature Protocols, Vol:5, Pages:1005-1018. PMID: 20448546

Beckonert O, Keun HC, Ebbels TMD, et al., 2007, Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extractsNature Protocols, Vol:2, Pages:2692-2703. PMID: 18007604